Recently generated high-throughput sequencing data sets have shed light on the important regulatory roles of noncoding RNA (ncRNA) molecules in the development of higher organisms. Nowadays it is well-known that regulatory ncRNAs can bind complementary RNA or DNA sequences and recruit chromatin remodelers to selectively modulate gene expression. Consequently, genome sequencing and transcriptomics technologies are now being used to reveal hidden associations among ncRNAs and distinct biological mechanisms. This is the case for the diamondback moth Plutella xylostella, a worldwide pest known to infest cruciferous crops and to display resistance to most insecticides, including Bacillus thuringiensis (Bt) based biopesticides. In P. xylostella, it is thought that ncRNAs could play important roles in both development and insecticide resistance. This review will highlight recent insights into the roles of ncRNAs in P. xylostella and related lepidopterans, and will outline genetic engineering technologies which might be used to design efficient ncRNA-based pest control strategies. This article is categorized under: Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs.

中文翻译：

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非编码RNA在杀虫剂耐受性方面的重要性日益凸显，特别是小菜蛾（鳞翅目：Plutellidae）。

最近生成的高通量测序数据集阐明了非编码RNA（ncRNA）分子在高级生物发展中的重要调控作用。如今，众所周知，调节性ncRNA可以结合互补的RNA或DNA序列并募集染色质重塑剂以选择性地调节基因表达。因此，基因组测序和转录组学技术现已用于揭示ncRNA与不同生物学机制之间的隐秘联系。小菜蛾小菜蛾（Plutella xylostella）就是这种情况，这是一种世界性害虫，已知会侵害十字花科作物并显示出对大多数杀虫剂的抗性，包括基于苏云金芽孢杆菌（Bt）的生物农药。在小菜蛾中，人们认为ncRNA在发育和杀虫剂抗性中都可能起重要作用。这篇综述将着重介绍关于ncRNA在小菜蛾和相关鳞翅目动物中的作用的最新见解，并将概述可用于设计基于ncRNA的有效害虫控制策略的基因工程技术。本文归类于：调节RNA / RNAi /核糖开关>调节RNA。