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Advances that facilitate the study of large RNA structure and dynamics by nuclear magnetic resonance spectroscopy.
WIREs RNA ( IF 7.3 ) Pub Date : 2019-04-25 , DOI: 10.1002/wrna.1541
Huaqun Zhang 1 , Sarah C Keane 1, 2
Affiliation  

The characterization of functional yet nonprotein coding (nc) RNAs has expanded the role of RNA in the cell from a passive player in the central dogma of molecular biology to an active regulator of gene expression. The misregulation of ncRNA function has been linked with a variety of diseases and disorders ranging from cancers to neurodegeneration. However, a detailed molecular understanding of how ncRNAs function has been limited; due, in part, to the difficulties associated with obtaining high-resolution structures of large RNAs. Tertiary structure determination of RNA as a whole is hampered by various technical challenges, all of which are exacerbated as the size of the RNA increases. Namely, RNAs tend to be highly flexible and dynamic molecules, which are difficult to crystallize. Biomolecular nuclear magnetic resonance (NMR) spectroscopy offers a viable alternative to determining the structure of large RNA molecules that do not readily crystallize, but is itself hindered by some technical limitations. Recently, a series of advancements have allowed the biomolecular NMR field to overcome, at least in part, some of these limitations. These advances include improvements in sample preparation strategies as well as methodological improvements. Together, these innovations pave the way for the study of ever larger RNA molecules that have important biological function. This article is categorized under: RNA Structure and Dynamics > RNA Structure, Dynamics, and Chemistry Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs RNA Structure and Dynamics > Influence of RNA Structure in Biological Systems.

中文翻译:

通过核磁共振波谱促进大RNA结构和动力学研究的进展。

功能性但非蛋白质编码(nc)RNA的表征已将RNA在细胞中的作用从分子生物学中心教条中的被动角色扩展到基因表达的主动调节剂。ncRNA功能的失调与多种疾病和失调有关,从癌症到神经退行性变。但是,对ncRNA的功能的详细分子理解受到限制。部分原因是与获得大RNA的高分辨率结构相关的困难。RNA整体的三级结构确定受到各种技术挑战的阻碍,随着RNA大小的增加,所有这些挑战都会加剧。即,RNA趋于是高度柔性和动态的分子,其难以结晶。生物分子核磁共振(NMR)光谱提供了一种确定大RNA分子结构的可行选择,该结构不易结晶,但本身受到一些技术限制。近来,一系列进展已经使生物分子NMR领域至少部分地克服了这些局限性。这些进步包括样品制备策略的改进以及方法上的改进。总之,这些创新为研究具有重要生物学功能的更大RNA分子铺平了道路。本文归类于:RNA结构和动力学> RNA结构,动力学和化学调节RNA / RNAi /核糖开关>调节RNA的RNA结构和动力学> RNA结构在生物系统中的影响。
更新日期:2019-11-01
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