To rapidly find “best-in-class” antibody therapeutics, it has become essential to develop high throughput (HTP) processes that allow rapid assessment of antibodies for functional and molecular properties. Consequently, it is critical to have access to sufficient amounts of high quality antibody, to carry out accurate and quantitative characterization. We have developed automated workflows using liquid handling systems to conduct affinity-based purification either in batch or tip column mode. Here, we demonstrate the capability to purify >2000 antibodies per day from microscale (1 mL) cultures. Our optimized, automated process for human IgG1 purification using MabSelect SuRe resin achieves ∼70% recovery over a wide range of antibody loads, up to 500 µg. This HTP process works well for hybridoma-derived antibodies that can be purified by MabSelect SuRe resin. For rat IgG2a, which is often encountered in hybridoma cultures and is challenging to purify via an HTP process, we established automated purification with GammaBind Plus resin. Using these HTP purification processes, we can efficiently recover sufficient amounts of antibodies from mammalian transient or hybridoma cultures with quality comparable to conventional column purification.

为了快速找到“同类最佳”的抗体疗法，开发高通量（HTP）流程已变得至关重要，该流程可快速评估抗体的功能和分子特性。因此，获取足够数量的高质量抗体以进行准确和定量的表征至关重要。我们已经开发了使用液体处理系统的自动化工作流程，以批处理或尖端色谱柱模式进行基于亲和力的纯化。在这里，我们展示了每天从微量（1 mL）培养物中纯化> 2000种抗体的能力。我们使用MabSelect SuRe树脂对人IgG1进行纯化的优化，自动化流程，可在高达500 µg的各种抗体负载范围内实现约70％的回收率。此HTP方法对于可通过MabSelect SuRe树脂纯化的杂交瘤来源抗体非常有效。对于在杂交瘤细胞培养中经常遇到且难以通过HTP流程纯化的大鼠IgG2a，我们建立了使用GammaBind Plus树脂的自动纯化方法。使用这些HTP纯化过程，我们可以从哺乳动物瞬时或杂交瘤培养物中有效回收足够量的抗体，其质量可与常规色谱柱纯化相比。