This study was designed to identify populations of Rhipicephalus sanguineus collected from Iran and also to study molecular taxonomy of Rhipicephalus species using cytochrome C oxidase subunit 1 (COI) and internal transcribed spacer 2 (ITS2) sequences. Tick specimens were collected from livestock (sheep and goat) in 14 Iranian provinces. DNA of individual specimens was extracted and PCR was done on these samples. So, 62 sequencing (33 COI and 29 ITS2) were done, successfully. Morphologically, we identified four Rhipicephalus species, namely R. bursa, R. sanguineus (s.l.), R. sanguineus (s.s.), and R. turanicus based on taxonomic keys. The data obtained from the phylogenetic analyses of COI and ITS2 fragments present a possible conflict regarding the identity of R. sanguineus species. Thus, the molecular identification of R. sanguineus group might be different according to mitochondrial and nuclear DNA. The results show a phylogenic conflict based on COI and ITS2 phylogeny in a tree topology. We dealt with three genetic entities in R. sanguineus group (i.e. R. sanguineus (s.s.), R. sanguineus (s.l.), and R. turanicus) based on COI phylogeny and two genetic clades (i.e. R. sanguineus (s.s.) and R. sanguineus (s.l.)/R. turanicus) according to ITS2 phylogeny.

这项研究旨在鉴定从伊朗收集的血吸虫，并利用细胞色素C氧化酶亚基1（COI）和内部转录间隔区2（ITS2）序列研究吸虫的分子分类学。Iran虫标本采集自伊朗14个省的牲畜（绵羊和山羊）。提取单个样品的DNA，并在这些样品上进行PCR。因此，成功完成了62个测序（33个COI和29个ITS2）。形态上，我们确定了四个扇头蜱种，即R.囊，血红扇头蜱（SL），血红扇头蜱（ss）和基于分类键的turanicus R. 从COI和ITS2片段的系统发育分析中获得的数据提出了关于红血球菌物种身份的可能冲突。因此，根据线粒体和核DNA ，血红红球菌基团的分子鉴定可能有所不同。结果表明，在树形拓扑结构中，基于COI和ITS2系统发育的系统发育冲突。我们在处理与三个遗传实体血红组（即血红扇头蜱（SS），血红扇头蜱（SL），以及R.图兰）根据根据ITS2系统发育，COI系统发育和两个遗传进化枝（即R. sanguineus（ss）和R. sanguineus（sl）/ R. turanicus）。