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Mitochondrial cytochrome oxidase (COI) sequence-based identification of pod borer of Thylacoptila sp. AA isolated from Senna alata (L.) Roxb. for the first time in India.
Mitochondrial DNA Part A ( IF 1.695 ) Pub Date : 2019-02-08 , DOI: 10.1080/24701394.2018.1532415
Savitha Veeraragavan 1 , Ramamoorthy Duraisamy 1 , Sudhakaran Mani 2
Affiliation  

In the present study, two pod borer larvae were found to damage pods of the host plant Senna alata. As pods (seeds) are commercially important, it prompted the investigators to find out the adult of pod borer larvae by molecular sequencing and reported in this article for the first time from S. alata. Successful amplification was achieved for chloroplast DNA isolated from leaves of host plant S. alata and tissue DNA from unknown larvae and sequenced. The sequences were submitted to NCBI domain, and the taxonomic position of host plant of pod borer larvae was confirmed as S. alata (L.) Roxb. Of the two sequences belonging to pod borer larvae, specimen 1 (S1) matched with already available three sequences of Thylacoptila sp. AA. But, specimen 2 (S2) showed significant variation in its genetic distance in Neighborhood Joining tree and maximum likelihood tree; these factors imply that specimen 2 (S2) is distinct from Thylacoptila sp. AA. Therefore, it is reported that specimen 2 may be represented as Thylacoptila sp. BB where in BB indicates variant from other available sequences. Nonetheless, reporting species of Thylacoptila as insect pest in pods of S. alata is an important contribution to the annals of insect–pest–plant interaction more importantly in medicinally important plant species S. alata.



中文翻译:

基于线粒体细胞色素氧化酶(COI)序列的thylacoptila sp荚bore的鉴定。从番泻叶(L.)Roxb分离的AA。第一次在印度。

在本研究中,发现了两个豆荚r幼虫破坏寄主植物番泻叶的豆荚。由于豆荚(种子)在商业上很重要,这促使研究人员通过分子测序找出了豆荚bore幼虫的成虫,并首次在阿拉善沙门氏菌中进行了报道。从宿主植物S. alata的叶中分离的叶绿体DNA和未知幼虫的组织DNA进行了成功的扩增,并进行了测序。将该序列提交给NCBI结构域,并确认荚幼虫的宿主植物的分类学位置为S. alata(L.)Roxb。在属于豆荚bore幼虫的两个序列中,标本1(S1)与已经获得的三个thythoptioptila序列匹配sp。机管局。但是,标本2(S2)在邻域连接树和最大似然树中的遗传距离显示出显着差异。这些因素意味着标本2(S2)与Thylacoptila sp。是不同的。机管局。因此,据报道,标本2可以被表示为thylacoptila sp。BB其中,BB中的BB表示来自其他可用序列的变体。尽管如此,将拟南芥的豆科植物报道S. alata豆荚中的害虫,这是对昆虫-害虫-植物相互作用史的重要贡献,在医学上重要的植物物种S. alata中更重要。

更新日期:2019-02-08
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