The increased cardiovascular risk in RA (rheumatoid arthritis) cannot be explained by common quantitative circulating lipid parameters. The objective of the study was to characterize the modifications in HDL phosphosphingolipidome in patients with RA to identify qualitative modifications which could better predict the risk for CVD. Nineteen patients with RA were compared to control subjects paired for age, sex, BMI, and criteria of metabolic syndrome. The characterization of total HDL phosphosphingolipidome was performed by LC-MS/MS. RA was associated with an increased HDL content of lysophosphatidylcholine and a decreased content of PC (phosphatidylcholine), respectively, positively and negatively associated with cardiovascular risk. A discriminant molecular signature composed of 18 lipids was obtained in the HDL from RA patients. The detailed analysis of phospholipid species showed that molecules carrying omega-3 FA (fatty acids), notably docosahexaenoic acid (C22:6 n-3), were depleted in HDL isolated from RA patients. By contrast, two PE (phosphatidylethanolamine) species carrying arachidonic acid (C20:4 n-6) were increased in HDL from RA patients. Furthermore, disease activity and severity indexes were associated with altered HDL content of 4 PE and 2 PC species. In conclusion, the composition of HDL phosphosphingolipidome is altered during RA. Identification of a lipidomic signature could therefore represent a promising biomarker for CVD risk. Although a causal link remains to be demonstrated, pharmacological and nutritional interventions targeting the normalization of the FA composition of altered phospholipids could help to fight against RA-related inflammation and CVD risk.

中文翻译：

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HDL颗粒磷脂成分的变化以及类风湿关节炎中炎症的作用。

RA（类风湿关节炎）的心血管风险增加无法通过常见的定量循环脂质参数来解释。这项研究的目的是鉴定RA患者HDL磷酸鞘脂组的修饰，以鉴定可以更好地预测CVD风险的定性修饰。将19例RA患者与年龄，性别，BMI和代谢综合征标准配对的对照组进行比较。通过LC-MS / MS进行总HDL磷酸鞘脂脂质体的表征。RA分别与溶血磷脂酰胆碱的HDL含量升高和PC（磷脂酰胆碱）含量降低相关，与心血管风险呈正相关和负相关。从RA患者的HDL中获得了由18种脂质组成的判别性分子标记。对磷脂种类的详细分析表明，从RA患者中分离出的HDL中耗尽了带有omega-3 FA（脂肪酸），尤其是二十二碳六烯酸（C22：6 n-3）的分子。相比之下，RA患者的HDL中携带花生四烯酸（C20：4 n-6）的两种PE（磷脂酰乙醇胺）种类增加。此外，疾病活动性和严重性指数与4种PE和2种PC种类的HDL含量变化有关。总之，在RA期间HDL磷酸鞘脂脂质体的组成发生改变。因此，脂质体特征的鉴定可以代表有前途的CVD风险生物标志物。尽管因果关系仍有待证明，