Background Nuclear pore complexes (NPCs) act as nano-turnstiles within nuclear membranes between the cytoplasm and nucleus of mammalian cells. NPC proteins, called nucleoporins (Nups), mediate trafficking of proteins and RNA into and out of the nucleus, and are involved in a variety of mitotic processes. We previously reported that Nup62 localizes to the centrosome and mitotic spindle during mitosis, and plays a role in centrosome homeostasis. However, whether Nup58, a Nup62 subcomplex protein, also localizes to spindle poles is unknown. Result Herein, we show that Nup58 localizes to the nuclear rim during interphase, and to mitotic spindles, centrosomes, and midbodies during mitosis. Our confocal microscopy, live-cell imaging, and stimulated emission depletion nanoscopy results also demonstrated that Nup58 localized to the centrosomes during metaphase and relocalized to midbodies during abscission. Depletion of Nup58 resulted in centrosomal abnormalities and delayed abscission. Conclusion Nup58 localized at the centrosomes and mitotic spindle during metaphase and relocalized at midbodies during abscission. This study highlights the important role of Nup58 in mitosis.

中文翻译：

---

核孔蛋白 Nup58 在有丝分裂期间定位于中心体和中间体。

背景 核孔复合物 (NPC) 在哺乳动物细胞的细胞质和细胞核之间的核膜内充当纳米旋转门。NPC 蛋白质，称为核孔蛋白 (Nups)，介导蛋白质和 RNA 进出细胞核的运输，并参与多种有丝分裂过程。我们之前报道过 Nup62 在有丝分裂过程中定位于中心体和有丝分裂纺锤体，并在中心体稳态中发挥作用。然而，Nup58（一种 Nup62 亚复合蛋白）是否也定位于纺锤极尚不清楚。结果 在此，我们表明 Nup58 在间期定位于核边缘，并在有丝分裂期间定位于有丝分裂纺锤体、中心体和中间体。我们的共聚焦显微镜、活细胞成像、和受激发射耗尽纳米镜检查结果还表明，Nup58 在中期定位于中心体，并在脱落期间重新定位到中间体。Nup58 的消耗导致中心体异常和延迟脱落。结论 Nup58 在中期定位于中心体和有丝分裂纺锤体，在脱落期间重新定位于中体。这项研究强调了 Nup58 在有丝分裂中的重要作用。