A manufacturing process for sheet-based stacked scaffolds (SSCs) based on laser-cutting (LC) was developed. The sheets consist of Polycaprolactone/Hydroxyapatite (PCL/HA) composite material. Single sheets were cut from a PCL/HA foil and stacked to scaffolds with interconnecting pores of defined sizes. HA quantities up to 50% were processable with high reproducibility, while the accuracy was dependent on the applied laser power. The smallest achievable pore sizes were about 40 µm, while the smallest stable solid structures were about 125 µm. The human mesenchymal stem cell line SCP-1 was cultured on the manufactured PCL/HA scaffolds. The cells developed a natural morphology and were able to differentiate to functional osteoblasts. The generation of PCL/HA SSCs via LC offers new possibilities for tissue engineering (TE) approaches. It is reliable and fast, with high resolution. The SSC approach allows for facile cell seeding and analysis of cell fate within the three-dimensional cell culture, thus allowing for the generation of functional tissue constructs.

中文翻译：

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一种基于激光切割的制造工艺，该工艺使用聚己内酯/羟基磷灰石复合聚合物生成用于组织工程的三维支架。

开发了基于激光切割（LC）的片状堆叠式支架（SSC）的制造工艺。片材由聚己内酯/羟基磷灰石（PCL / HA）复合材料组成。从PCL / HA箔上切下单张纸，然后堆叠到具有确定大小的互连孔的支架上。HA量高达50％的样品具有很高的重现性，而加工精度取决于所施加的激光功率。可达到的最小孔径约为40 µm，而最小的稳定固体结构约为125 µm。在制造的PCL / HA支架上培养人间充质干细胞系SCP-1。这些细胞发展出自然的形态，能够分化为功能性成骨细胞。通过LC生成PCL / HA SSC为组织工程（TE）方法提供了新的可能性。它是可靠且快速的，具有高分辨率。SSC方法允许在3维细胞培养物中方便地进行细胞播种和细胞命运分析，从而允许生成功能性组织构建体。