The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells. The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites. The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days. Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined. Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44^high cells after both freezing regimens was found. Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used. Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44⁺ and increased CD24⁺ cells. None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44^high tumor cells. The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.

中文翻译：

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不同的冷冻保存方案对艾氏癌生长的影响。

冷冻速率是决定使用低温的目的的决定性因素，即低温用于肿瘤细胞的冷冻消融或冷冻库。研究目的是确定不同的冷冻保存方案对体内艾氏癌（EC）生长和所形成的腹水亚群组成的影响。将先前以慢速和快速速率冷冻保存的EC细胞在小鼠的腹膜腔（PC）中培养7天。使用CD44和CD24标记通过流式细胞仪确定PC中的绝对细胞数，肿瘤亚群组成。升温后，EC亚群组成立即大量重新分布，致癌性^最高的CD44含量降低找到两种冷冻方案后的细胞。体内培养7天有助于EC亚群组成的恢复，但是当使用缓慢冷却时，肿瘤生长强度有所降低。快速冷却促进了肿瘤生长的抑制显著与CD44的减少的数目⁺和CD24增加⁺细胞。冷冻保存方案均未导致完全消除致肿瘤的CD44^高肿瘤细胞。冷冻速率决定了EC亚群组成的保存及其在体内的生长强度。