BACKGROUND Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). RESULTS The LG1 strain, which grew equally well in the two conditions, was found to express a set of proteins related to cellular processes vital for survival, as well as proteins involved in adaptation and tolerance to Mn (II). The multicopper oxidase CueO was identified, indicating its probable participation in the Mn (II) bio-oxidation; however, its expression was not modulated by the presence of Mn (II). A set of proteins related to cell and metabolic processes vital to the cells were downregulated in the presence of Mn (II), while cell membrane-related proteins involved in the maintenance of cell integrity and survival under stress were upregulated under this condition. CONCLUSIONS These findings indicate that the LG1 strain may be applied successfully in the bioremediation of Mn (II), and the shotgun approach provides an efficient means for obtaining the total proteome of this species.

中文翻译：

---

锰对粘质沙雷氏菌蛋白质组学组成的影响（II）。

背景技术蛋白质组学是研究重金属胁迫下微生物动态生理反应的重要工具。为了深入了解细菌如何响应锰（II）并鉴定涉及Mn（II）氧化的蛋白质，将the弹枪蛋白质组学方法应用于可能存在的Mn（II）氧化的粘质沙雷氏菌菌株，该菌株在缺乏和存在Mn的条件下培养（II）。结果发现，在两种条件下生长均相同的LG1菌株表达了一组与生存过程至关重要的细胞过程相关的蛋白质，以及与Mn适应性和耐受性有关的蛋白质。鉴定出多铜氧化酶CueO，表明其可能参与了Mn（II）生物氧化；但是，其表达不受Mn（II）的存在调节。在Mn（II）的存在下，一组与细胞和对细胞至关重要的代谢过程相关的蛋白质被下调，而在这种条件下，与维持细胞完整性和应激生存相关的细胞膜相关蛋白质被上调。结论这些发现表明，LG1菌株可以成功地应用于Mn（II）的生物修复中，并且approach弹枪方法为获得该物种的总蛋白质组提供了有效的手段。