BACKGROUND Large-scale processing of lignocellulosics for glucose production generally relies on high temperature and acidic or alkaline conditions. However, extreme conditions produce chemical contaminants that complicate downstream processing. A method that mainly rely on mechanical and enzymatic reaction completely averts such problem and generates unmodified lignin. Products from this process could find novel applications in the chemicals, feed and food industry. But a large-scale system suitable for this purpose is yet to be developed. In this study we applied simultaneous enzymatic saccharification and communition (SESC) for the pre-treatment of a representative lignocellulosic biomass, cedar softwood, under both laboratory and large-scale conditions. RESULTS Laboratory-scale comminution achieved a maximum saccharification efficiency of 80% at the optimum pH of 6. It was possible to recycle the supernatant to concentrate the glucose without affecting the efficiency. During the direct alcohol fermentation of SESC slurry, a high yield of ethanol was attained. The mild reaction conditions prevented the generation of undesired chemical inhibitors. Large-scale SESC treatment using a commercial beads mill system achieved a saccharification efficiency of 60% at an energy consumption of 50 MJ/kg biomass. CONCLUSION SESC is very promising for the mild and clean processing of lignocellulose to generate glucose and unmodified lignin in a large scale. Economic feasibility is highly dependent on its potential to generate high value natural products for energy, specialty chemicals, feed and food application.

中文翻译：

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同时进行酶促糖化和粉碎，以使木质纤维素生物质朝着天然产物的价值增加。

背景技术用于生产葡萄糖的木质纤维素的大规模加工通常依赖于高温和酸性或碱性条件。但是，极端条件会产生化学污染物，使下游加工复杂化。一种主要依靠机械和酶促反应的方法可以完全避免此类问题，并生成未修饰的木质素。通过此过程获得的产品可以在化学，饲料和食品工业中找到新颖的应用。但是，尚未开发出适合于此目的的大规模系统。在这项研究中，我们在实验室和大规模条件下，应用同步酶促糖化和酶解（SESC）预处理代表性的木质纤维素生物质雪松软木。结果实验室规模的粉碎在最适pH值为6时达到了80％的最大糖化效率。可以回收上清液以浓缩葡萄糖而不影响效率。在SESC浆液的直接乙醇发酵过程中，获得了高产率的乙醇。温和的反应条件阻止了不希望的化学抑制剂的产生。使用商业珠磨机系统的大规模SESC处理在50 MJ / kg生物质的能耗下实现了60％的糖化效率。结论SESC对于轻柔和清洁的木质纤维素生产大规模产生葡萄糖和未修饰的木质素的前景非常广阔。经济可行性在很大程度上取决于其产生高价值天然产物以用于能源，特种化学品，