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Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator
Insect Science ( IF 4 ) Pub Date : 2019-08-06 , DOI: 10.1111/1744-7917.12711
Olga Volovych 1, 2 , Zhe Lin 1 , Jie Du 1, 2 , Hong Jiang 1 , Zhen Zou 1, 2
Affiliation  

Recently, parasitoid wasp species Microplitis mediator has evoked increasing research attention due to its possible use in the control of Lepidoptera insects. Because insect development involves changes in cuticle composition, identification and expression analysis of M. mediator cuticular proteins may clarify the mechanisms involved in parasite development processes. We found 70 cuticular proteins from the M. mediator transcriptome and divided them into seven distinct families. Expression profiling indicated that most of these cuticular protein genes have expression peaks specific for one particular developmental stage of M. mediator. Eggs and pupae have the highest number of transcriptionally active cuticular protein genes (47 and 52 respectively). Only 12 of these genes maintained high expression activity during late larval development. Functional analysis of two larval proteins, MmCPR3 and MmCPR14, suggested their important role in the proper organization of the cuticle layers of larvae. During M. mediator larval development, normal cuticle formation can be supported by a limited number of cuticular proteins.

中文翻译:

内寄生蜂、Microplitis mediator 角质层蛋白的鉴定和时间表达谱

最近,寄生蜂物种 Microplitis mediator 由于其可能用于控制鳞翅目昆虫而引起了越来越多的研究关注。由于昆虫发育涉及角质层组成的变化,介导微球菌角质层蛋白的鉴定和表达分析可能会阐明寄生虫发育过程中涉及的机制。我们从 M. mediator 转录组中发现了 70 种角质蛋白,并将它们分为七个不同的家族。表达谱表明,大多数这些表皮蛋白基因具有针对介导分枝杆菌的一个特定发育阶段特异的表达峰。卵和蛹具有最多数量的转录活性角质层蛋白基因(分别为 47 和 52)。这些基因中只有 12 个在幼虫发育后期保持高表达活性。对两种幼虫蛋白 MmCPR3 和 MmCPR14 的功能分析表明,它们在幼虫角质层的正确组织中发挥着重要作用。在介体分枝杆菌幼虫发育过程中,正常的角质层形成可由有限数量的角质层蛋白支持。
更新日期:2019-08-06
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