Major Glioblastoma's hallmarks include proliferation, invasion and heterogeneity. Biological 3D tumor spheroid models can serve as intermediate systems between traditional 2D cell culture and complex in vivo models. Tumor spheroids have been shown to more accurately reproduce the spatial organization and microenvironmental factors of in vivo micro-tumors, such as relevant gradients of nutrients and other molecular agents, while they maintain cell-to-cell and cell-to-matrix interactions. In vitro 3D assays are useful to monitor these properties. Here, we test the suitability of the well-known T98 G Glioblastoma cell line in such a 3D assay. The doubling time and death rate parameters of T98 G are estimated, as well as their spheroidal growth-expansion curves with and without the presence of basement membrane substrate. The T98 G invasive profile is characterized by collective morphology and proliferation-associated invasion. We show that the T98 G secondary GB cell line exhibits both invasive and proliferative capabilities in 3D and thus, can serve as control cell line for the 3D in vitro study of primary GB cell cultures.

中文翻译：

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T98G胶质母细胞瘤细胞系表型表征的3D肿瘤球体模型。

胶质母细胞瘤的主要特征包括增殖，侵袭和异质性。生物3D肿瘤球体模型可以充当传统2D细胞培养与复杂体内模型之间的中间系统。肿瘤球体已被证明可以更准确地再现体内微肿瘤的空间组织和微环境因素，例如营养物和其他分子试剂的相关梯度，同时它们还能维持细胞间和细胞间的相互作用。体外3D分析可用于监视这些属性。在这里，我们在这种3D分析中测试了众所周知的T98 G胶质母细胞瘤细胞系的适用性。估计T98 G的倍增时间和死亡率参数，以及在存在和不存在基底膜基底的情况下其球状体的生长-膨胀曲线。T98 G侵袭性特征是集体形态和与增殖相关的侵袭。我们显示，T98 G继发性GB细胞系在3D中展示侵袭和增殖能力，因此，可以用作原始GB细胞培养物的3D体外研究的对照细胞系。