The value of the zebrafish (Danio rerio) as a model organism continues to expand. In developing the model, current feeding practice in zebrafish laboratories includes the use of commercially available diets. In this study, we compared outcomes in growth, body composition, and reproduction among zebrafish fed five highly utilized commercial diets and one formulated chemically defined reference diet. Wild-type zebrafish larvae were raised on live feed until 21 days postfertilization and then fed diets for 16 weeks. All fish received a daily ration of >5% of body weight (adjusted biweekly). Growth varied among diets throughout the feeding trial, and at study termination (week 16), significant differences among diets were observed for terminal weight gain, body condition index, body fat deposition, and reproductive outcomes. In addition, the proportion of viable embryos produced from females fed the formulated reference diet was high relative to the commercial diets. These data suggest that metabolic profiles, most likely reflecting nutrient/energy availability, utilization, and allocation, vary relative to diet in zebrafish. Undefined differences in metabolic profiles could result in erroneous predictions of health outcomes and make comparisons among laboratories more challenging. We recommend that dietary standards should be defined for zebrafish to support their common utility in biomedical research.

中文翻译：

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商业和实验室饮食对斑马鱼Danio rerio的生长，身体成分和繁殖的影响。

斑马鱼（Danio rerio）作为模型生物的价值不断扩大。在开发该模型时，斑马鱼实验室目前的饲养方法包括使用市售的饲料。在这项研究中，我们比较了饲喂五种高度商业化饮食和一种化学成分确定的参考饮食的斑马鱼在生长，身体成分和繁殖方面的结局。用活饲料饲养野生型斑马鱼幼虫，直到受精后21天，然后喂食16周。所有鱼的日配给量均大于体重的5％（每两周调整一次）。在整个喂养试验中，饮食之间的生长情况各不相同，并且在研究结束时（第16周），饮食之间的终末体重增加，身体状况指数，身体脂肪沉积和生殖结果之间存在显着差异。此外，相对于商业饮食，从配制参考饮食中喂养的雌性产生的有生命的胚胎比例很高。这些数据表明，代谢谱（最有可能反映出营养/能量的利用，利用和分配）相对于斑马鱼的饮食有所不同。代谢曲线的不确定差异可能导致对健康结局的错误预测，并使实验室之间的比较更具挑战性。我们建议应该为斑马鱼定义饮食标准，以支持它们在生物医学研究中的通用性。代谢曲线的不确定差异可能导致对健康结局的错误预测，并使实验室之间的比较更具挑战性。我们建议应该为斑马鱼定义饮食标准，以支持它们在生物医学研究中的通用性。代谢曲线的不确定差异可能导致对健康结局的错误预测，并使实验室之间的比较更具挑战性。我们建议应该为斑马鱼定义饮食标准，以支持它们在生物医学研究中的通用性。