Amines function as neuromodulators throughout the animal kingdom. In decapod crustaceans, the amines serving neuromodulatory roles include dopamine, octopamine, serotonin and histamine. While much work has focused on examining the physiological effects of amines on decapod nervous systems, the identity of the native enzymes involved in their biosynthesis remains largely unknown. In an attempt to help fill this void, a transcriptome generated from multiple portions of the crab, Cancer borealis, nervous system, a species that has long served as a model species for investigating the neuromodulatory control of rhythmically active neural networks, was used to identify putative amine biosynthetic enzyme-encoding transcripts, and by proxy, proteins. Transcripts encoding full complements of the enzymes involved in the production of dopamine, octopamine, serotonin, and histamine were deduced from the C. borealis assembly, i.e., tryptophan–phenylalanine hydroxylase, tyrosine hydroxylase, DOPA decarboxylase, tyrosine decarboxylase, tyramine β-hydroxylase, tryptophan hydroxylase, and histidine decarboxylase. All proteins deduced from the C. borealis transcripts appear to be full-length sequences, with reciprocal BLAST and structural domain analyses supporting the protein family annotations ascribed to them. These data provide the first descriptions of the native amine biosynthetic enzymes of C. borealis, and as such, serve as a resource for initiating gene-based studies of aminergic control of physiology and behavior at the level of biosynthesis in this important biomedical model.

中文翻译：

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鉴定蟹的神经系统中的假定胺生物合成酶。

胺在整个动物界中均作为神经调节剂。在十足纲甲壳动物中，起到神经调节作用的胺包括多巴胺，章鱼胺，5-羟色胺和组胺。尽管许多工作集中在研究胺对十足动物神经系统的生理影响，但参与其生物合成的天然酶的身份仍然未知。在试图帮助填补这一空白，从螃蟹的多个部分生成的转录组，癌症北欧化工神经系统，一种长期以来一直是研究节律性活动神经网络的神经调节控制的模型物种的物种，已被用来识别推定的胺生物合成酶编码的转录本，并通过代理来鉴定蛋白质。编码完整编码与多巴胺，章鱼胺，5-羟色胺和组胺生产有关的酶的转录物，是从北方衣原体大会上推导出的，即色氨酸-苯丙氨酸羟化酶，酪氨酸羟化酶，DOPA脱羧酶，酪氨酸脱羧酶，酪胺β-羟化酶，色氨酸羟化酶和组氨酸脱羧酶。从北极衣藻推断出的所有蛋白质转录本似乎是全长序列，相互的BLAST和结构域分析支持了归因于它们的蛋白质家族注释。这些数据提供了北美衣原体天然胺生物合成酶的第一个描述，因此，可作为资源在这一重要的生物医学模型中启动基于基因的生理学和行为学方面的胺能控制的研究资源。