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Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy.
Biotechniques ( IF 2.7 ) Pub Date : 2019-3-15 , DOI: 10.2144/btn-2018-0106
Zhi Li 1 , Yael Zilberman 1 , Qing-Bin Lu 2 , Xiaowu Shirley Tang 1
Affiliation  

An electrochemical approach was devised for detecting DNA damage and differentiating two DNA damage mechanisms, which is important to the design of new chemotherapeutics. This approach combined two platforms, based on the detection of base damage and DNA strand cleavage. In this work, our approach was demonstrated for the detection of cisplatin-induced DNA damage and the enhancement effects of two electron donors, N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) and reduced graphene oxide (rGO). Our results demonstrated that TMPD enhanced DNA strand cleavage, supporting the proposed dissociative electron transfer mechanism. While rGO, which is an efficient electron donor, failed to show any enhancement (suggesting the lack of free-radical generation), overall, this electrochemical approach could be implemented for discovering next-generation DNA damage-based chemotherapy drugs.

中文翻译:

探测DNA损伤机理和设计基于顺铂的联合化疗的电化学方法。

设计了一种电化学方法来检测DNA损伤并区分两种DNA损伤机理,这对于设计新的化学疗法很重要。该方法基于碱基损伤和DNA链断裂的检测,结合了两个平台。在这项工作中,我们的方法被证明可用于检测顺铂引起的DNA损伤以及两个电子供体N,N,N',N'-四甲基对苯二胺(TMPD)和氧化石墨烯(rGO)的增强作用。 )。我们的结果表明,TMPD增强了DNA链的裂解,支持了拟议的解离电子转移机制。总体而言,尽管rGO是高效的电子供体,但未显示出任何增强作用(建议缺乏自由基生成),
更新日期:2020-08-21
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