Hydrogen-producing acetogens (HPA) have a transitional role in anaerobic wastewater treatment. Thus, bioaugmentation with HPA cultures can enhance the chemical oxygen demand (COD) removal efficiency and CH₄ yield of anaerobic wastewater treatment. Cultures with high degradation capacities for propionic acid and butyric acid were obtained through continuous subculture in enrichment medium and were designated as Z08 and Z12. Bioaugmentation with Z08 and Z12 increased CH₄ production by glucose removal to 1.58. Bioaugmentation with Z08 and Z12 increased the COD removal rate in molasses wastewater from 71.60% to 85.84%. The specific H₂ and CH₄ yields from COD removal increased by factors of 1.54 and 1.63, respectively. Results show that bioaugmentation with HPA-dominated cultures can improve CH₄ production from COD removal. Furthermore, hydrogen-producing acetogenesis was identified as the rate-limiting step in anaerobic wastewater treatment.

中文翻译：

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混合产氢乙酸菌的生物强化可提高糖蜜废水处理中的甲烷产量。

产氢乙酸菌（HPA）在厌氧废水处理中具有过渡作用。因此，HPA培养物的生物增强可以提高厌氧废水处理的化学需氧量(COD)去除效率和CH _4产量。通过在增菌培养基中连续传代培养，获得对丙酸和丁酸具有高降解能力的培养物，命名为Z08和Z12。Z08和Z12的生物增强通过葡萄糖去除将CH ₄产量增加至1.58。Z08和Z12的生物强化将糖蜜废水中COD的去除率从71.60%提高到85.84%。COD 去除的 H ₂和 CH ₄比产量分别增加了 1.54 和 1.63 倍。结果表明，以 HPA 为主的培养物进行生物强化可以提高COD 去除中的CH _4产量。此外，产氢乙酸作用被确定为厌氧废水处理的限速步骤。