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Evaluation of white Kwao Krua (Pueraria candollei Grah. ex Benth.) products sold in Thailand by molecular, chemical, and microscopic analyses.
Journal of Natural Medicines ( IF 3.3 ) Pub Date : 2019-08-03 , DOI: 10.1007/s11418-019-01351-2
Aekkhaluck Intharuksa 1 , Masashi Kitamura 2 , Nichakan Peerakam 3 , Wannaree Charoensup 1 , Hirokazu Ando 4 , Yohei Sasaki 4 , Panee Sirisa-Ard 1
Affiliation  

The tuberous roots of Pueraria candollei Grah. ex Benth. (Fabaceae), commonly known as white Kwao Krua, are used to relieve menopausal symptoms in Thai traditional medicine because they contain phytoestrogens. Black and red Kwao Krua crude drugs exist as well, but they have different botanical origins and pharmacological activities. There is a high demand for white Kwao Krua products, but because of the limited availability of the plant material, it is suspected that the adulteration and misidentification of white Kwao Krua crude drugs and products occur. In this study, we authenticated white Kwao Krua products collected from Thai herbal markets by molecular, chemical, and microscopic analyses. The nucleotide sequences in the internal transcribed spacer (ITS) and trnH–psbA regions of 23 samples of authentic P. candollei were analyzed, and both regions were found to have intraspecific DNA polymorphisms. Based on the single nucleotide polymorphisms in the ITS1 region, species-specific primer sets of P. candollei were designed to authenticate white Kwao Krua and differentiate it from red and black Kwao Krua. Only the PCR products of KWP02 were not amplified by the primer sets. Isoflavonoid contents and microscopic features were used to support the results of molecular analysis to clarify the botanical origin of white Kwao Krua. Molecular, chemical and microscopic methods confirmed that all the Thai Kwao Krua products examined in this study contained authentic “white Kwao Krua” as claimed on their labels.

中文翻译:

通过分子,化学和微观分析,对在泰国销售的白色夸瓜(Pueraria candollei Grah。ex Benth。)产品进行评估。

葛根的块茎根。前Benth。(Fabaceae),通常称为白色Kwao Krua,因其含有植物雌激素,可用于缓解泰国传统医学中的更年期症状。也有黑色和红色的Kwao Krua原料药,但是它们具有不同的植物来源和药理活性。人们对白色Kwao Krua产品有很高的需求,但是由于植物原料的供应有限,怀疑白色Kwao Krua原料药和产品的掺假和误认会发生。在这项研究中,我们通过分子,化学和显微镜分析对从泰国草药市场收集的白色Kwao Krua产品进行了鉴定。内部转录间隔区(ITS)和trn H– psb中的核苷酸序列分析了真实的加拿大假单胞菌的23个样品的区域,并且发现两个区域都具有种内DNA多态性。基于ITS1区的单核苷酸多态性,设计了P. candollei的物种特异性引物组以鉴定白色Kwao Krua并将其与红色和黑色Kwao Krua区分开。仅KWP02的PCR产物没有被引物组扩增。异黄酮的含量和微观特征被用于支持分子分析的结果,以阐明白色夸奥克鲁亚的植物起源。分子,化学和显微镜方法证实,本研究中检验的所有Thai Kwao Krua产品均带有标签上所标明的真实“白色Kwao Krua”。
更新日期:2019-08-03
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