Chikungunya virus (CHIKV) is primarily transmitted by Aedes spp. mosquitoes. The present study investigated vector competence for CHIKV in Aedes aegypti and Aedes albopictus mosquitoes found in Madurai, South India. The role of receptor proteins on midguts contributing to permissiveness of CHIKV to Aedes spp. mosquitoes was also undertaken. Mosquitoes were orally infected with CHIKV DRDE-06. Infection of midguts and dissemination to heads was confirmed by immunofluorescence assay at different time points. A plaque assay was performed from mosquito homogenates at different time points to study CHIKV replication. Presence of putative CHIKV receptor proteins on mosquito midgut epithelial cells was detected by virus overlay protein binding assay (VOPBA). The identity of these proteins was established using mass spectrometry. CHIKV infection of Ae. aegypti and Ae. albopictus midguts and dissemination to heads was observed to be similar. A plaque assay performed with infected mosquito homogenates revealed that CHIKV replication dynamics was similar in Aedes sp. mosquitoes until 28 days post infection. VOPBA performed with mosquito midgut membrane proteins revealed that prohibitin could serve as a putative CHIKV receptor on Aedes mosquito midguts, whereas an absence of CHIKV binding protein/s on Culex quinquefasciatus midguts can partially explain the non-permissiveness of these mosquitoes to infection.

中文翻译：

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了解基孔肯雅病毒载体在三种蚊子中的能力机制。

基孔肯雅病毒（CHIKV）主要由伊蚊（Aedes spp）传播。蚊子。本研究调查了在印度南部马杜赖发现的埃及伊蚊和白纹伊蚊中CHIKV的载体能力。受体蛋白在中肠上的作用有助于CHIKV对伊蚊的允许。蚊子也被捕了。蚊子被CHIKV DRDE-06口服感染。通过免疫荧光测定在不同时间点确认中肠的感染和向头部的传播。从蚊子匀浆在不同时间点进行噬斑测定以研究CHIKV复制。通过病毒覆盖蛋白结合测定（VOPBA）检测蚊中肠上皮细胞上是否存在假定的CHIKV受体蛋白。这些蛋白质的身份是通过质谱法确定的。CHIKV感染的Ae。埃及和埃及。观察到白带中肠和向头部的传播相似。用感染的蚊子匀浆进行的噬斑测定显示，在伊蚊中CHIKV复制动力学相似。直到感染后28天才有蚊子。用蚊子中肠膜蛋白进行的VOPBA结果显示，禁止素可以作为伊蚊蚊子中的一个假定的CHIKV受体，而西洋参库克斯蚊子中肠中不存在CHIKV结合蛋白可以部分解释这些蚊子对感染的不容许性。