Physical exercise can improve gait, balance, tremor, flexibility, grip strength and motor coordination in Parkinson’s disease (PD) patients. Several lines of evidence have also shown the therapeutic potential of dietary management and supplementation in halting the progression of PD. However, there is a lack of research on the combined effects of physical activity and nutrition in the progression of PD. We test the effects exercise and dietary modification in a Drosophila model of PD. In this study, we fed Drosophila parkin mutants high protein and high carbohydrate diets without and with stearic acid (4 treatments in total). In parallel, we subjected mutants to a regimen of exercise using a purpose-built ‘Power tower’ exercise machine. We then measured climbing ability, aconitase activity, and basal mitochondrial ROS levels. We observed that exercising parkin mutants fed the high protein diet improved their climbing ability and increased aconitase activity. There was an additional improvement in climbing and aconitase activity in exercised parkin mutants fed the high protein diet supplemented with stearic acid. No benefits of exercise were seen in parkin mutants fed the high carbohydrate diet. Combined, these results suggest that dietary management along with physical activty has potential to improve mitochondrial biogenesis and delay the progression of PD in Drosophila parkin mutants.

体育锻炼可以改善帕金森病（PD）患者的步态，平衡，震颤，柔韧性，抓地力和运动协调性。几条证据还显示，饮食管理和补充疗法可以阻止PD的发展。然而，缺乏关于体育活动和营养对PD进展的综合影响的研究。我们在果蝇PD模型中测试运动和饮食修改的影响。在这项研究中，我们饲喂果蝇帕金不使用硬脂酸和使用硬脂酸的高蛋白和高碳水化合物饮食的突变体（总共4种治疗方法）。同时，我们使用专用的“ Power Tower”锻炼机对突变体进行了锻炼。然后，我们测量了攀爬能力，乌头酸酶活性和基底线粒体ROS水平。我们观察到，以高蛋白饮食喂养的运动型帕金突变体改善了其攀爬能力并增加了乌头酸酶活性。用补充了硬脂酸的高蛋白饮食喂养的运动型帕金突变体，其攀爬和乌头酸酶活性有了进一步的改善。在帕金中没有发现运动的好处突变者以高碳水化合物饮食为食。结合起来，这些结果表明，饮食管理以及身体活动具有改善果蝇帕金突变体中线粒体生物发生并延缓PD进程的潜力。