Maturation conditions and oocytes quality have substantial roles on developmental competence of unreconstructed or reconstructed oocytes. Cloning has been reported successfully with low efficiency through embryonic or somatic nuclear transfer into enucleated metaphase II oocytes. It has been suggested that introducing embryonic or somatic nucleus to cytoplast at earlier stage might improve reprogramming of the introduced nucleus. In addition, the synchronization between the donor nucleus and recipient cytoplasts might effect on reprogramming and further embryonic development. Therefore, the question arises; does the cytoplast of germinal vesicle (GV) oocyte containing nuclear sap improve developmental competence upon reconstruction with embryonic/somatic nucleus compared with MII cytoplast. It has been indicated that GV material is essential for remodeling of sperm or embryonic or somatic nucleus in GV oocyte cytoplast and their further embryonic developmental competence. GV cytoplasts could be obtained through micromanipulation. Different micromanipulation techniques of immature oocytes at different stages were adapted in addition to introducing donor nuclei at G0/G1, S and G2/M phase, and enucleolation technique as well. Upon micromanipulation, it could obtain GV cytoplasts; cumulus-free and without GV material, cumulus-complexes and without GV material, cumulus-free and with GV material, and cumulus-complexes and with GV material in addition to enucleolated GV oocytes. Therefore, this short review will address briefly the importance of maturation conditions, cumulus cells, oocyte quality, the techniques of enucleation GV oocyte, the cell cycle stage of the introduced donor cell, or nucleus for oocyte maturation and embryo development.

中文翻译：

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胚泡/体核重建后，胚泡卵母细胞的细胞质和核物质是否支持发育能力。

成熟状态和卵母细胞质量对未重建或重建的卵母细胞的发育能力具有重要作用。已经成功地报道了通过胚胎或体细胞核转移到去核的中期II卵母细胞中的低效率的成功克隆。已经提出在较早阶段将胚核或体细胞核引入细胞质可改善所引入核的重编程。另外，供体核和受体细胞质之间的同步可能影响重编程和进一步的胚胎发育。因此，出现了问题。与MII细胞质相比，含有胚泡的生殖小泡（GV）卵母细胞的细胞质能改善胚胎/体细胞核的发育能力吗？已经表明，GV材料对于GV卵母细胞胞质中精子或胚胎或体细胞核的重塑及其进一步的胚胎发育能力是必不可少的。GV细胞质可以通过显微操作获得。除了在G0 / G1，S和G2 / M期引入供体核外，还采用了不同的阶段，对不同阶段的未成熟卵母细胞进行了不同的显微操作技术，以及进行了核酸化技术。经过微操作，可以获得GV细胞质。无积云，无GV物质，积云，无GV物质，无积云，有GV物质，积云，无GV物质，以及去核化的GV卵母细胞。因此，这篇简短的评论将简要讨论成熟条件，卵丘细胞，卵母细胞质量，去核GV卵母细胞技术的重要性，