A simple, rapid, and efficient liquid chromatography tandem mass spectrometry (LC–MS/MS) method, operated in electrospray ionization and quadrupole linear ion trap modes, has been developed for the identification and structural characterization of aflatoxins in peanuts and its derivative products or bean sauce. Samples (5 g) were extracted with acetonitrile/water/formic acid (79:20:1, v/v). After centrifugation and dilution, the extracts were separated on a C18 analytical column by gradient elution (acetonitrile/0.2% formic acid) and analyzed by UPLC–MS/MS. External calibration was used for qualification. The developed multiple reaction monitoring–information-dependent acquisition–enhanced product ion method enabled quantification and confirmation of the analytes in a single run. Enhanced product ion mode was used for qualitative analysis, while multiple reaction monitoring mode was used for quantitative analysis. An in-house library was constructed for identification. Calibration curves showed good linearity with correlation coefficients (r) higher than 0.994. Limits of detection were determined to be below 0.26 µg kg−1 for most analytes. The recoveries for those substances were in the acceptable range of 80.2%–119.1%. A new LC–MS3 method was established for further confirmation. One pickled pepper peanut was found to contain aflatoxins B1, B2, and G1 with contents of 90.93, 26.64, and 1.92 µg kg−1, respectively.

中文翻译：

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在多反应监测、增强型子离子扫描和 MS3 模式下使用 LC-MS/MS QTRAP 系统对四种黄曲霉毒素进行定量和确认

一种简单、快速、高效的液相色谱串联质谱 (LC-MS/MS) 方法，在电喷雾电离和四极杆线性离子阱模式下运行，已开发用于鉴定和结构表征花生及其衍生产品中的黄曲霉毒素或豆酱。样品 (5 g) 用乙腈/水/甲酸 (79:20:1, v/v) 萃取。离心和稀释后，通过梯度洗脱（乙腈/0.2% 甲酸）在 C18 分析柱上分离提取物，并通过 UPLC-MS/MS 进行分析。外部校准用于鉴定。开发的多反应监测-依赖于信息的采集-增强型子离子方法能够在单次运行中对分析物进行定量和确认。增强型子离子模式用于定性分析，同时采用多反应监测模式进行定量分析。建造了一个内部图书馆用于识别。校准曲线显示出良好的线性，相关系数 (r) 高于 0.994。大多数分析物的检测限低于 0.26 µg kg-1。这些物质的回收率在 80.2%–119.1% 的可接受范围内。建立了一种新的 LC-MS3 方法以进行进一步确认。一颗腌辣椒花生被发现含有黄曲霉毒素 B1、B2 和 G1，其含量分别为 90.93、26.64 和 1.92 µg kg−1。大多数分析物为 26 µg kg−1。这些物质的回收率在 80.2%–119.1% 的可接受范围内。建立了一种新的 LC-MS3 方法以进行进一步确认。一颗腌辣椒花生被发现含有黄曲霉毒素 B1、B2 和 G1，其含量分别为 90.93、26.64 和 1.92 µg kg−1。大多数分析物为 26 µg kg−1。这些物质的回收率在 80.2%–119.1% 的可接受范围内。建立了一种新的 LC-MS3 方法以进行进一步确认。一颗腌辣椒花生被发现含有黄曲霉毒素 B1、B2 和 G1，其含量分别为 90.93、26.64 和 1.92 µg kg−1。