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Fine mapping a quantitative trait locus, qSER-7, that controls stigma exsertion rate in rice (Oryza sativa L.).
Rice ( IF 5.5 ) Pub Date : 2019-07-11 , DOI: 10.1186/s12284-019-0304-z Yi Liu 1, 2 , Anning Zhang 1, 2 , Feiming Wang 2 , Deyan Kong 2 , Mingshou Li 2 , Junguo Bi 2 , Fenyun Zhang 2 , Jiahong Wang 2 , Xingxing Luo 2 , Zhongquan Pan 2 , Xinqiao Yu 2 , Guolan Liu 2 , Lijun Luo 1, 2
Rice ( IF 5.5 ) Pub Date : 2019-07-11 , DOI: 10.1186/s12284-019-0304-z Yi Liu 1, 2 , Anning Zhang 1, 2 , Feiming Wang 2 , Deyan Kong 2 , Mingshou Li 2 , Junguo Bi 2 , Fenyun Zhang 2 , Jiahong Wang 2 , Xingxing Luo 2 , Zhongquan Pan 2 , Xinqiao Yu 2 , Guolan Liu 2 , Lijun Luo 1, 2
Affiliation
BACKGROUND
Stigma exsertion rate (SER) is a key determinant of outcrossing in hybrid rice seed production. A quantitative trait locus (QTL) for stigma exsertion rate in rice, qSER-7, has previously been detected on chromosome 7 by using a F2 population derived from two indica cytoplasmic male sterility (CMS) maintainers, Huhan 1B and II-32B.
RESULTS
The chromosomal location of qSER-7 was precisely delimited by fine-scale mapping. Near-isogenic lines (NILs) were established, one of which isolated the locus in the qSER-7II-32B line, which contains an introgressed segment of II-32B in the Huhan 1B genetic background, and exhibits a significantly higher stigma exsertion rate than that of the recurrent parent. Using 3192 individuals from the BC4F2 segregation population, the QTL qSER-7 was narrowed down to a 28.4-kb region between the markers RM3859 and Indel4373 on chromosome 7. According to the rice genome annotation database, three genes were predicted within the target region. Real-time PCR analysis showed significantly higher expression levels of LOC_Os07g15370 and LOC_Os07g15390 in II-32B than in Huhan 1B. LOC_Os07g15370(OsNRAMP5) was a previously reported gene for Mn and Cd transporter. The stigma exertion rates of OsNRAMP5-overexpressing plants were significantly higher than that of wild type plants, in contrast, a T-DNA insertion mutant osnramp5 showed a lower stigma exertion rate.
CONCLUSIONS
In the present study, the QTL qSER-7 was isolated to a region between the markers RM3859 and Indel4373. Two candidate genes were selected based on the expression difference between the two parents, which can facilitate the further cloning of the gene underlying the quantitative trait associated with qSER-7 as well as the marker-assisted transfer of desirable genes for stigma exsertion rate improvement in rice.
中文翻译：
精细定位一个定量性状基因座qSER-7，该基因座控制水稻（Oryza sativa L.）的柱头外露率。
背景技术柱头外露率（SER）是杂交水稻种子生产中异交的关键决定因素。水稻的柱头外露率定量性状基因座（QTL），qSER-7，以前是通过使用来自两个d型雄性不育（CMS）维持基因Huhan 1B和II-32B的F2群体检测到的。结果qSER-7的染色体位置已通过精细比例图精确界定。建立了近等基因系（NILs），其中之一分离出qSER-7II-32B系中的基因座，该系在武汉1B遗传背景中包含II-32B的一个渐渗片段，并且柱头显着高于经常性的父母。使用BC4F2隔离种群中的3192个个体，将QTL qSER-7缩小到28个。位于7号染色体上的标记RM3859和Indel4373之间的4 kb区域。根据水稻基因组注释数据库，在目标区域内预测了三个基因。实时PCR分析显示，II-32B中的LOC_Os07g15370和LOC_Os07g15390的表达水平明显高于Huhan 1B。LOC_Os07g15370（OsNRAMP5）是先前报道的Mn和Cd转运蛋白基因。OsNRAMP5过表达植物的柱头使用率显着高于野生型植物，相比之下，T-DNA插入突变体osnramp5显示较低的柱头使用率。结论在本研究中，将QTL qSER-7分离到标记RM3859和Indel4373之间的区域。根据两个亲本之间的表达差异选择两个候选基因，
更新日期：2019-11-01
中文翻译：
精细定位一个定量性状基因座qSER-7，该基因座控制水稻（Oryza sativa L.）的柱头外露率。
背景技术柱头外露率（SER）是杂交水稻种子生产中异交的关键决定因素。水稻的柱头外露率定量性状基因座（QTL），qSER-7，以前是通过使用来自两个d型雄性不育（CMS）维持基因Huhan 1B和II-32B的F2群体检测到的。结果qSER-7的染色体位置已通过精细比例图精确界定。建立了近等基因系（NILs），其中之一分离出qSER-7II-32B系中的基因座，该系在武汉1B遗传背景中包含II-32B的一个渐渗片段，并且柱头显着高于经常性的父母。使用BC4F2隔离种群中的3192个个体，将QTL qSER-7缩小到28个。位于7号染色体上的标记RM3859和Indel4373之间的4 kb区域。根据水稻基因组注释数据库，在目标区域内预测了三个基因。实时PCR分析显示，II-32B中的LOC_Os07g15370和LOC_Os07g15390的表达水平明显高于Huhan 1B。LOC_Os07g15370（OsNRAMP5）是先前报道的Mn和Cd转运蛋白基因。OsNRAMP5过表达植物的柱头使用率显着高于野生型植物，相比之下，T-DNA插入突变体osnramp5显示较低的柱头使用率。结论在本研究中，将QTL qSER-7分离到标记RM3859和Indel4373之间的区域。根据两个亲本之间的表达差异选择两个候选基因，
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