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Growth and cellular patterning during fetal human inner ear development studied by a correlative imaging approach.
BMC Developmental Biology ( IF 1.978 ) Pub Date : 2019-05-20 , DOI: 10.1186/s12861-019-0191-y
Lejo Johnson Chacko 1 , David Wertjanz 1 , Consolato Sergi 2 , Jozsef Dudas 1 , Natalie Fischer 1 , Theresa Eberharter 1 , Romed Hoermann 3 , Rudolf Glueckert 1, 4 , Helga Fritsch 3 , Helge Rask-Andersen 5 , Anneliese Schrott-Fischer 1 , Stephan Handschuh 6
Affiliation  

BACKGROUND Progressive transformation of the otic placode into the functional inner ear during gestational development in humans leads to the acquisition of hearing perception via the cochlea and balance and spatial orientation via the vestibular organ. RESULTS Using a correlative approach involving micro-computerized tomography (micro-CT), transmission electron microscopy and histological techniques we were able to examine both the morphological and cellular changes associated with human inner ear development. Such an evaluation allowed for the examination of 3D geometry with high spatial and temporal resolution. In concert with gestational progression and growth of the cochlear duct, an increase in the distance between some of the Crista ampullaris is evident in all the specimens examined from GW12 to GW36. A parallel increase in the distances between the macular organs - fetal utricle and saccule - is also evident across the gestational stages examined. The distances between both the utricle and saccule to the three cristae ampullares also increased across the stages examined. A gradient in hair cell differentiation is apparent from apex to base of the fetal cochlea even at GW14. CONCLUSION We present structural information on human inner ear development across multiple levels of biological organization, including gross-morphology of the inner ear, cellular and subcellular details of hearing and vestibular organs, as well as ultrastructural details in the developing sensory epithelia. This enabled the gathering of detailed information regarding morphometric changes as well in realizing the complex developmental patterns of the human inner ear. We were able to quantify the volumetric and linear aspects of selected gestational inner ear specimens enabling a better understanding of the cellular changes across the fetal gestational timeline. Moreover, these data could serve as a reference for better understanding disorders that arise during inner ear development.

中文翻译:

通过相关成像方法研究胎儿人内耳发育过程中的生长和细胞模式。

背景技术在人的妊娠发育过程中,耳部斑块逐渐转变为功能性内耳导致通过耳蜗获得听力知觉,并通过前庭器官获得平衡和空间定向。结果使用涉及微计算机断层扫描(micro-CT),透射电子显微镜和组织学技术的相关方法,我们能够检查与人内耳发育相关的形态学和细胞变化。这样的评估允许检查具有高空间和时间分辨率的3D几何。与妊娠进程和耳蜗管的生长相一致,在从GW12到GW36检查的所有标本中,一些Crista壶腹之间的距离明显增加。在检查的整个妊娠阶段中,黄斑器官之间的距离(胎儿的囊和囊突)也平行增加。在检查的各个阶段中,囊和囊与三个tric壶壶腹之间的距离也增加了。即使在GW14,从胎儿耳蜗的根尖到根部,毛细胞分化的梯度也是明显的。结论我们介绍了跨多个生物组织水平的人类内耳发育的结构信息,包括内耳的总体形态,听力和前庭器官的细胞和亚细胞细节,以及正在发展的感觉上皮细胞的超微结构细节。这样就可以收集有关形态变化的详细信息,以及实现人类内耳的复杂发育模式。我们能够量化选定的妊娠内耳标本的体积和线性方面,从而更好地了解整个胎儿妊娠时间轴上的细胞变化。此外,这些数据可作为参考,以更好地了解内耳发育过程中出现的疾病。
更新日期:2020-04-22
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