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Comparative chromosome painting in Columbidae (Columbiformes) reinforces divergence in Passerea and Columbea.
Chromosome Research ( IF 2.6 ) Pub Date : 2018-06-09 , DOI: 10.1007/s10577-018-9580-5
Rafael Kretschmer 1, 2 , Ivanete de Oliveira Furo 3 , Ricardo José Gunski 4 , Analía Del Valle Garnero 4 , Jorge C Pereira 2 , Patricia C M O'Brien 2 , Malcolm A Ferguson-Smith 2 , Edivaldo Herculano Corrêa de Oliveira 5, 6 , Thales Renato Ochotorena de Freitas 1
Affiliation  

Pigeons and doves (Columbiformes) are one of the oldest and most diverse extant lineages of birds. However, the karyotype evolution within Columbiformes remains unclear. To delineate the synteny-conserved segments and karyotypic differences among four Columbidae species, we used chromosome painting from Gallus gallus (GGA, 2n = 78) and Leucopternis albicollis (LAL, 2n = 68). Besides that, a set of painting probes for the eared dove, Zenaida auriculata (ZAU, 2n = 76), was generated from flow-sorted chromosomes. Chromosome painting with GGA and ZAU probes showed conservation of the first ten ancestral pairs in Z. auriculata, Columba livia, and Columbina picui, while in Leptotila verreauxi, fusion of the ancestral chromosomes 6 and 7 was observed. However, LAL probes revealed a complex reorganization of ancestral chromosome 1, involving paracentric and pericentric inversions. Because of the presence of similar intrachromosomal rearrangements in the chromosomes corresponding to GGA1q in the Columbidae and Passeriformes species but without a common origin, these results are consistent with the recent proposal of divergence within Neoaves (Passerea and Columbea). In addition, inversions in chromosome 2 were identified in C. picui and L. verreauxi. Thus, in four species of distinct genera of the Columbidae family, unique chromosomal rearrangements have occurred during karyotype evolution, confirming that despite conservation of the ancestral syntenic groups, these chromosomes have been modified by the occurrence of intrachromosomal rearrangements.

中文翻译:

哥伦比科(Columbiformes)的比较染色体绘画增强了Passerea和哥伦布亚的分歧。

鸽子和鸽子(Columbiformes)是现存鸟类中最古老和最多样化的世系之一。但是,哥伦比目内的核型进化仍不清楚。为了描绘四种哥伦比科物种之间的节律保守段和核型差异,我们使用了来自鸡(Galus gallus)(GGA,2n = 78)和白粉蝶(Lucopternis albicollis,LAL,2n = 68)的染色体绘画。除此之外,还从流分类的染色体中生成了一组斑鸠的绘画探针(ZAUida auriculata,ZAU,2n = 76)。用GGA和ZAU探针进行的染色体涂漆显示了Z. auriculata,Columba livia和Columbina picui中前十对祖先对的保守,而在Leptotila verreauxi中,观察到祖先的6号和7号染色体融合。但是,LAL探针显示了祖先染色体1的复杂重组 涉及顺心和逆心反转。由于在Columbidae和Passeriformes物种中对应于GGA1q的染色体中存在相似的染色体内重排,但没有共同的起源,所以这些结果与Neoaves(Passerea和Columbea)最近提出的分歧一致。此外,在C. picui和L. verreauxi中鉴定了2号染色体的倒位。因此,在哥伦比科的四个不同属中,在染色体核型进化过程中发生了独特的染色体重排,这证实了尽管保留了祖先的同族基团,但这些染色体已通过发生染色体内重排而被修饰。由于在Columbidae和Passeriformes物种中对应于GGA1q的染色体中存在相似的染色体内重排,但没有共同的起源,所以这些结果与Neoaves(Passerea和Columbea)最近提出的分歧一致。此外,在C. picui和L. verreauxi中鉴定了2号染色体的倒位。因此,在哥伦比科的四个不同属中,在染色体核型进化过程中发生了独特的染色体重排,这证实了尽管保留了祖先的同族基团,但这些染色体已通过发生染色体内重排而被修饰。由于在Columbidae和Passeriformes物种中对应于GGA1q的染色体中存在相似的染色体内重排,但没有共同的起源,所以这些结果与Neoaves(Passerea和Columbea)最近提出的分歧一致。另外,在C. picui和L. verreauxi中鉴定了2号染色体的倒位。因此,在哥伦比科家族的四个不同属中,在核型进化过程中发生了独特的染色体重排,这证实了尽管保留了祖先的同族基团,但这些染色体已经通过染色体内重排而被修饰。这些结果与Neoaves(Passerea和Columbea)内部最近的分歧建议相一致。此外,在C. picui和L. verreauxi中鉴定了2号染色体的倒位。因此,在哥伦比科的四个不同属中,在染色体核型进化过程中发生了独特的染色体重排,这证实了尽管保留了祖先的同族基团,但这些染色体已经通过染色体内重排而被修饰。这些结果与Neoaves(Passerea和Columbea)内部最近的分歧建议相一致。此外,在C. picui和L. verreauxi中鉴定了2号染色体的倒位。因此,在哥伦比科的四个不同属中,在染色体核型进化过程中发生了独特的染色体重排,这证实了尽管保留了祖先的同族基团,但这些染色体已经通过染色体内重排而被修饰。
更新日期:2019-11-01
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