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Proinflammatory effects of dust storm and thermal inversion particulate matter (PM10) on human peripheral blood mononuclear cells (PBMCs) in vitro: a comparative approach and analysis.
Journal of Environmental Health Science and Engineering ( IF 3.4 ) Pub Date : 2019-04-18 , DOI: 10.1007/s40201-019-00362-1
Zahra Atafar 1 , Zahra Pourpak 2 , Masud Yunesian 1, 3 , Mohammad Hossein Nicknam 4, 5 , Mohammad Sadegh Hassanvand 6 , Narjes Soleimanifar 4 , Shiva Saghafi 2 , Zahra Alizadeh 2 , Soheila Rezaei 1 , Maryam Ghanbarian 1 , Mohammad Ghanbari Ghozikali 7 , Alvaro R Osornio-Vargas 8 , Kazem Naddafi 1, 6
Affiliation  

Particulate matter (PM) as the carcinogenic air pollutants can lead to aggravated health outcomes. Epidemiological studies demonstrated that PM can be engaged in different diseases such as cardiovascular, respiratory and cancer. The in vitro secretion of proinflammatory cytokines by human peripheral blood mononuclear cells (PBMCs) has been used to assess the effects of PM with an aerodynamic diameter < 10 μm (PM10). This study compared the proinflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1-beta (IL1-β) secretions of PBMCs exposed to PM10 of dust storm and inversion. We collected PM10 samples during the spring and autumn seasons in two locations. Isolated PBMCs were exposed separately to 50, 150, and 300 μg/ml of different type of PM10 for 4 and 24 h. The mean concentrations of TNF-α for the PM of dust storm and inversion were 6305.61 ± 2421 and 6651.74 ± 2820, respectively. Also the mean concentrations of IL1-β for the PM of dust storm and inversion were 556.86 ± 162 and 656.35 ± 196, respectively. Furthermore, these values for the production of IL-6 were 12,655 ± 5661 and 16,685 ± 8069, respectively. Although no significant difference was observed between the PM of dust storm and that of inversion with regard to PBMCs, the results showed a significant increase in the proinflammatory cytokine secretion of both PMs compared with the controls. Moreover, TNF-α, IL1-β, and IL-6 secreted in cells exposed to PM10 of dust storm were about 10 times more than the controls, these values for cells exposed to PM10 of inversion were around 10, 12, and 14 times more than the controls, respectively. It can be concluded that the PM10 of both dust storm and inversion can play a significant role in proinflammatory cytokine secretion due to its harmful effect on human health.
This picture shows the Proinflammatory cytokine producing potential of PM10 with two sources (dust storm and urban air pollution) in exposure with human PBMCs in vitro.


中文翻译:

沙尘暴和热转化颗粒物(PM10)对人外周血单个核细胞(PBMC)的促炎作用:比较方法和分析。

颗粒物(PM)作为致癌性空气污染物可导致健康状况恶化。流行病学研究表明,PM可能与多种疾病有关,例如心血管,呼吸道疾病和癌症。人类外周血单核细胞(PBMC)在体外分泌促炎细胞因子已被用于评估空气动力学直径<10μm(PM 10)的PM的影响。这项研究比较了暴露于沙尘暴和倒置PM 10的PBMC的促炎细胞因子肿瘤坏死因子α(TNF-α),白介素6(IL-6)和白介素1-β(IL1-β)分泌。我们收集了10号在春季和秋季两个地方采样。分离的PBMC分别暴露于50、150和300μg/ ml的不同类型的PM 10持续4和24小时。沙尘暴和反演的PM的TNF-α平均浓度分别为6305.61±2421和6651.74±2820。沙尘暴和反演的PM的平均IL1-β浓度分别为556.86±162和656.35±196。此外,IL-6产生的这些值分别为12655±5661和16685±8069。尽管在沙尘暴的PM和倒转的PM之间,在PBMC方面未观察到显着差异,但结果表明,与对照相比,两个PM的促炎细胞因子分泌显着增加。此外,暴露于沙尘暴的PM 10的细胞中分泌的TNF-α,IL1-β和IL-6约为对照的10倍,这是暴露于PM 10的细胞的这些值倒数分别是对照的10倍,12倍和14倍。可以得出结论,沙尘暴和反演的PM 10均由于其对人体健康的有害作用而在促炎性细胞因子分泌中起重要作用。
这张图片显示了在人PBMC体外暴露时,有两种来源(沙尘暴和城市空气污染)的PM 10的促炎细胞因子产生潜力。
更新日期:2019-04-18
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