We constructed a high-density genetic map for Mongolian gerbils (Meriones unguiculatus). We genotyped 137 F2 individuals with a genotype-by-sequencing (GBS) approach at over 10,000 loci and built the genetic map using a two-step approach. First, we chose the highest-quality set of 485 markers to construct a robust map of 1239 cM with 22 linkage groups as expected from the published karyotype. Second, we added an additional 5449 markers onto the map based on their genotype similarity with the original markers. We used the final marker set to assemble 1140 genomic scaffolds (containing ~ 20% of annotated genes) into a chromosome-level assembly. We used both genetic linkage and relative sequencing coverage in males and females to identify X- and Y-chromosome scaffolds and from these we designed a robust and internally-controlled PCR assay to determine sex. This assay will facilitate early stage sex-typing of embryonic and young gerbils which is difficult using current visual methods. Accession ID: Meriones unguiculatus: 10047.

中文翻译：

---

沙鼠的高密度遗传图谱和分子性别分型测定。

我们构建了蒙古沙鼠（Meriones unguiculatus）的高密度遗传图谱。我们采用基因型测序 (GBS) 方法对 10,000 多个位点的 137 个 F2 个体进行了基因分型，并使用两步方法构建了遗传图谱。首先，我们选择了最高质量的 485 个标记集来构建 1239 cM 的稳健图谱，其中包含 22 个连锁群，正如已发布的核型所预期的那样。其次，我们根据其与原始标记的基因型相似性在图谱上添加了额外的 5449 个标记。我们使用最终的标记集将 1140 个基因组支架（包含约 20% 的注释基因）组装成染色体水平组装。我们利用男性和女性的遗传连锁和相对测序覆盖率来识别 X 和 Y 染色体支架，并据此设计了一种强大的内部控制 PCR 测定法来确定性别。该测定将有助于胚胎和幼年沙鼠的早期性别分型，这使用当前的视觉方法是困难的。登记号：长爪沙鼠：10047。