Following an injury or resection, the mammalian liver has the capacity to regain its former volume and functioning by restoring itself. Studies have demonstrated that antioxidants play a role in hepatic regeneration. This study investigated the effect of 4-(3,4-dihydroxybenzoyloxymethyl) phenyl-O-β-D-glucopyranoside (PG) obtained from Origanum micranthum on liver regeneration. Sixty Wistar Albino rats were used. In the sham-operated group, a midline abdominal laparotomy was performed without hepatectomy. In the partial hepatectomy (PHx) group, the median and left lateral lobes were removed. Rats in the PHx group received 20 mg/kg/day PG intraperitoneally before being sacrificed at 24, 48, and 72 hrs, and 7 days later. Liver tissues were collected for immunohistochemical analysis and electron microscopic evaluation. We found an increase in mitotic index, and the numbers of Ki-67 stained hepatocytes in all PHx early stage groups (24 hr, 48hr, 72 hr), but not in 7-day groups. The regeneration mediators eNOS, iNOS, TNF-α and NF-κB were shown to increase in PHx groups. This increase was more prominent dependening on time. In the PHx treatment (PHx+PG) groups, while eNOS was still high, iNOS, TNF-α and NF-κB had decreased. The apoptotic index was markedly high in the PHx groups; this was prevented by PG treatment. These findings were supported by the ultrastructural results. Our findings indicate that PG supports liver regeneration, hepatocyte proliferation, reduced liver damage, and inflammatory mediators following PHx.

中文翻译：

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4-（3,4-二羟基苯甲酰氧基甲基）苯基-O-β-D-吡喃葡萄糖苷在肝脏再生中的作用。

受伤或切除后，哺乳动物的肝脏具有恢复自身容量并通过恢复自身功能的能力。研究表明，抗氧化剂在肝再生中起作用。这项研究调查了从牛至草制成的4-（3,4-二羟基苯甲酰氧基甲基）苯基-O-β-D-吡喃葡萄糖苷（PG）对肝脏再生的影响。使用60只Wistar Albino大鼠。假手术组未行肝切除术进行中线腹部剖腹手术。在部分肝切除术（PHx）组中，去除了中叶和左外侧叶。PHx组的大鼠腹膜内接受20 mg / kg / day PG，然后在24、48和72小时以及7天后处死。收集肝组织用于免疫组织化学分析和电子显微镜评估。我们发现所有PHx早期组（24小时，48小时，72小时）的有丝分裂指数和Ki-67染色的肝细胞数量均增加，但在7天组中则没有。PHx组的再生介质eNOS，iNOS，TNF-α和NF-κB升高。这种增加取决于时间。在PHx治疗（PHx + PG）组中，尽管eNOS仍然很高，但iNOS，TNF-α和NF-κB却降低了。在PHx组中，细胞凋亡指数显着高。这是通过PG治疗预防的。这些发现得到超微结构结果的支持。我们的发现表明，PG支持PHx后的肝再生，肝细胞增殖，减少的肝损伤和炎症介质。PHx组的再生介质eNOS，iNOS，TNF-α和NF-κB升高。这种增加取决于时间。在PHx治疗（PHx + PG）组中，尽管eNOS仍然很高，但iNOS，TNF-α和NF-κB却降低了。在PHx组中，细胞凋亡指数显着高。这是通过PG治疗预防的。这些发现得到超微结构结果的支持。我们的发现表明，PG支持PHx后的肝再生，肝细胞增殖，减少的肝损伤和炎症介质。PHx组的再生介质eNOS，iNOS，TNF-α和NF-κB升高。这种增加取决于时间。在PHx治疗（PHx + PG）组中，尽管eNOS仍然很高，但iNOS，TNF-α和NF-κB却降低了。在PHx组中，细胞凋亡指数显着高。这是通过PG治疗预防的。这些发现得到超微结构结果的支持。我们的发现表明，PG支持PHx后的肝再生，肝细胞增殖，减少的肝损伤和炎症介质。这是通过PG治疗预防的。这些发现得到超微结构结果的支持。我们的发现表明，PG支持PHx后的肝再生，肝细胞增殖，减少的肝损伤和炎症介质。这是通过PG治疗预防的。这些发现得到超微结构结果的支持。我们的发现表明，PG支持PHx后的肝再生，肝细胞增殖，减少的肝损伤和炎症介质。