Liver fibrosis is an excessive accumulation of scar tissue resulting from inflammation and cell death. Thioacetamide (TAA) is a well-known hepatotoxin that induces liver fibrosis. A marker of injured hepatocytes is transforming growth factor-beta 1 (TGF-β1), while alpha-smooth muscle actin (α-SMA) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are markers of activated hepatic stellate cells. Alpha-mangostin, a major xanthone derivative from the mangosteen pericarp, has been shown to have anti-oxidant and anti-inflammatory activities. The objective of this study was to determine whether alpha-mangostin has a protective effect on TAA-induced liver fibrosis in rats. The rats were treated by intraperitoneal injection of compounds for eight weeks. For the control group a mixture of dimethyl sulfoxide and phosphate buffered saline was administered. Two hundred mg/kg BW of TAA was administered three times weekly. Alpha-mangostin was administered at 5 mg/kg BW and silymarin at 100 mg/kg BW, both twice weekly. TAA induced histologically recognizable liver damage and fibrosis, as anticipated. Furthermore, it increased immunohistochemically detectable TGF-β1, α-SMA and TIMP-1. Co-administration of alpha-mangostin or silymarin with TAA prevented or ameliorated the effects of TAA administration alone. The anti-fibrotic effect of alpha-mangostin was stronger than that of silymarin.

中文翻译：

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如形态功能分析所揭示的，α-芒果素对硫代乙酰胺诱导的大鼠肝纤维化的保护作用。

肝纤维化是由炎症和细胞死亡导致的瘢痕组织的过度积累。硫代乙酰胺（TAA）是一种众所周知的肝毒素，可诱导肝纤维化。受损肝细胞的标志物是转化生长因子-β1（TGF-β1），而α平滑肌肌动蛋白（α-SMA）和金属蛋白酶1组织抑制剂（TIMP-1）是活化的肝星状细胞的标志物。Alman-mangostin是山竹果皮的主要黄酮酮衍生物，已被证明具有抗氧化和消炎作用。这项研究的目的是确定α-Mangostin对TAA诱导的大鼠肝纤维化是否具有保护作用。通过腹膜内注射化合物治疗大鼠八周。对于对照组，施用二甲基亚砜和磷酸盐缓冲盐水的混合物。每周三次给予200 mg / kg体重的TAA。每周两次以5 mg / kg BW的剂量施用Alman-Mangostin和以100 mg / kg BW的水飞蓟素施用。如预期的那样，TAA诱导了组织学上可识别的肝损伤和纤维化。此外，它增加了免疫组织化学可检测的TGF-β1，α-SMA和TIMP-1。α-芒果或水飞蓟素与TAA的共同给药可预防或改善TAA单独给药的效果。α-Mangostin的抗纤维化作用比水飞蓟素更强。α-SMA和TIMP-1。α-芒果或水飞蓟素与TAA的共同给药可预防或改善TAA单独给药的效果。α-Mangostin的抗纤维化作用比水飞蓟素更强。α-SMA和TIMP-1。α-芒果或水飞蓟素与TAA的共同给药可预防或改善TAA单独给药的效果。α-Mangostin的抗纤维化作用比水飞蓟素更强。