The wheat and barley CBF14 genes have been newly defined as key components of the light quality-dependent regulation of the freezing tolerance by the integration of phytochrome-mediated light and temperature signals. To further investigate the wavelength dependence of light-induced CBF14 expression in cereals, we carried out a detailed study using monochromatic light treatments at an inductive and a non-inductive temperature. Transcript levels of CBF14 gene in winter wheat Cheyenne, winter einkorn G3116 and winter barley Nure genotypes were monitored. We demonstrated that (1) CBF14 is most effectively induced by blue light and (2) provide evidence that this induction does not arise from light-controlled CRY gene expression. (3) We demonstrate that temperature shifts induce CBF14 transcription independent of the light conditions and that (4) the effect of temperature and light treatments are additive. Based on these data, it can be assumed that temperature and light signals are relayed to the level of CBF14 expression via separate signalling routes.

中文翻译：

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小麦和大麦中 CBF14 基因表达水平的光和温度信号

通过整合光敏色素介导的光和温度信号，小麦和大麦 CBF14 基因被新定义为耐寒性的光质依赖性调节的关键组成部分。为了进一步研究谷物中光诱导 CBF14 表达的波长依赖性，我们在感应和非感应温度下使用单色光处理进行了详细研究。监测了冬小麦夏安、冬单粒小麦 G3116 和冬大麦 Nure 基因型中 CBF14 基因的转录水平。我们证明 (1) CBF14 最有效地被蓝光诱导，(2) 提供证据表明这种诱导不是由光控制的 CRY 基因表达引起的。(3) 我们证明温度变化独立于光照条件诱导 CBF14 转录，并且 (4) 温度和光照处理的影响是相加的。基于这些数据，可以假设温度和光信号通过单独的信号通路传递到 CBF14 表达水平。