Due to the increasing incidence of fungal opportunistic infections and emergence of antibiotic‐resistant fungal strains, antimicrobial peptides (AMPs) are considered as ideal candidates for antifungal compounds. In silico methods can reduce the limitations of natural AMPs such as toxicity and instability and improve their antimicrobial properties and selectivity. In this study, we designed AurH1, a new truncated peptide, based on the six‐amino acid sequence of Aurein1.2. Further, the antimicrobial activities and toxicity effects of AurH1 on human skin fibroblast cells and red blood cells were investigated. Finally, field emission scanning electron microscopy (FE‐SEM) and flow cytometry were performed in order to study the mechanism of action of AurH1. The results indicated that AurH1 had only antifungal activity (at a minimal inhibitory concentration (MIC) of 7.3‐125 μg/mL) without any antibacterial effects on the selected bacteria, while Aurein1.2 had both antifungal and antibacterial activities as positive control. Furthermore, AurH1 did not show any toxicity on Hu02 cells and human red blood cells at its MIC range. In conclusion, it became clear that AurH1 is a selective peptide against fungi with no toxic effects on the selected bacteria and human cells.

中文翻译：

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AurH1：一种新的七肽，衍生自Aurein1.2抗菌肽，具有特定的排他性杀真菌活性。

由于真菌机会性感染的发生率不断增加，并且出现了抗生素抗性真菌菌株，因此抗菌肽（AMPs）被认为是抗真菌化合物的理想候选药物。计算机方法可以减少天然AMP的局限性，例如毒性和不稳定性，并提高其抗菌性能和选择性。在这项研究中，我们基于Aurein1.2的六氨基酸序列设计了新的截短肽AurH1。此外，研究了AurH1对人皮肤成纤维细胞和红细胞的抗菌活性和毒性作用。最后，为了研究AurH1的作用机理，进行了场发射扫描电子显微镜（FE-SEM）和流式细胞术。结果表明，AurH1仅具有抗真菌活性（最小抑菌浓度（MIC）为7.3-125μg/ mL），对所选细菌没有任何抗菌作用，而Aurein1.2作为阳性对照具有抗真菌和抗菌活性。此外，AurH1在其MIC范围内对Hu02细胞和人红细胞没有任何毒性。总之，很明显，AurH1是一种针对真菌的选择性肽，对选定的细菌和人类细胞没有毒性作用。