The low permeability of porin channels is the possible reason for Gram-negative bacterial resistance to antibiotics. The adaptive accumulation of lysophosphatidylethanolamine (LPE) in Yersinia pseudotuberculosis induces conformational changes of OmpF porin that may hinder the transport of antibiotics through this channel. The present study was aimed to test whether the changes in LPE content affect the resistance of bacteria to ampicillin. The addition of glucose to the culture medium was shown to simultaneously increase the level of LPE and minimum inhibitory concentration (MIC) for ampicillin of Y. pseudotuberculosis cells 6- and 2-fold, respectively. However, the coadministration of glucose and polyphenol extract from buckwheat husks reduced the content of LPE 2-fold and restored MIC to the control value. Thus, PBEH can be used as antibiotic adjuvant to improve an antibiotic's ability to cross the outer membrane. The present work demonstrated: (i) the role of adaptive changes in the lipid composition of Y. pseudotuberculosis in the development of antibiotic resistance, and (ii) the promising use of PBEH in combination therapy to increase the susceptibility of Gram-negative bacteria to the conventional β-lactam antibiotics, probably attenuating in vivo a previously demonstrated effect of LPE on the conformation and function of the OmpF channel.

中文翻译：

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细菌信封中溶血磷脂酰乙醇胺含量的适应性变化与假结核耶尔森氏菌氨苄青霉素敏感性之间的关系。

孔蛋白通道的低渗透性是革兰氏阴性细菌对抗生素产生耐药性的可能原因。溶血磷脂酰乙醇胺（LPE）在假性耶尔森氏菌中的适应性积累会诱导OmpF孔蛋白的构象变化，这可能会阻止抗生素通过此通道的运输。本研究旨在测试LPE含量的变化是否会影响细菌对氨苄西林的耐药性。已显示向培养基中添加葡萄糖可同时提高假结核耶尔森氏菌细胞的LPE水平和氨苄青霉素的最小抑制浓度（MIC）分别为6倍和2倍。然而，葡萄糖和荞麦皮中多酚提取物的共同给药使LPE的含量降低了2倍，并使MIC恢复到控制值。从而，PBEH可用作抗生素佐剂，以提高抗生素穿过外膜的能力。目前的工作表明：（i）假结核耶尔森氏菌脂质组成的适应性变化在抗生素耐药性发展中的作用；（ii）PBEH在联合治疗中有望增加革兰氏阴性菌对细菌的敏感性传统的β-内酰胺类抗生素可能会减轻体内先前证明的LPE对OmpF通道构象和功能的影响。