We developed new PCR assays that target beta-tubulin (TUB2) and 14 alpha-demethylase (CYP51) genes and used them for the species-specific detection of Blumeria graminis f. sp. tritici (Bgt). Based on fungi DNA sequences available in the NCBI (National Center for Biotechnology Information) GenBank database we developed simplex and duplex PCR assays. The specificities of the primer sets were evaluated using environmental samples of wheat leaves collected during the 2015/2016 growing season across Poland. Primer sets LidBg17/18 and LidBg21/22 strongly amplified fragments of the expected length for all 67 tested samples. Primer specificity was confirmed using field samples of Zymoseptoria tri-tici, Puccinia triticina (syn. P. recondita f. sp. tritici), P. striiformis f. sp. tritici, and Pyrenophora tritici-repentis.

中文翻译：

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一种检测鸡蛋白粉病新PCR方法的开发与应用。sp。小麦。

我们开发了针对β-微管蛋白（TUB2）和14个α-脱甲基酶（CYP51）基因的新PCR检测方法，并将其用于Blumeria graminis f的物种特异性检测。sp。小麦（Bgt）。基于NCBI（国家生物技术信息中心）GenBank数据库中可用的真菌DNA序列，我们开发了单重和双重PCR检测方法。使用波兰/ 2015/2016生长季收集的小麦叶片环境样品评估了引物组的特异性。引物对所有67个受测样品都设置了预期长度的LidBg17 / 18和LidBg21 / 22强扩增片段。引物特异性已用三面体Zymoseptoria tici，Puccinia triticina（syn。P. recondita f。sp。tritici），P。striiformis f。的田间样品证实。sp。小麦和小麦黑斑病菌。