Fluoro-Jade is a fluorescein-derived fluorochrome which specifically binds to damaged neurons. Due to this characteristic, it is commonly used for the histochemical detection and quantification of neurodegeneration in mounted brain sections. Here, we describe an alternative and simpler histochemistry protocol based on the use of free-floating brain sections. For this purpose, we have used brain slices from wild-type and 5xFAD mice as well as from mice that received an intracerebral injection of oligomeric amyloid beta peptides. We observed that our histochemistry staining procedure allows for a well-defined labeling of degenerating neurons providing a better signal-to-noise ratio staining than the commonly used one. In addition, our modified protocol demonstrates the ability of Fluoro-Jade C to also fluorescently label amyloid beta plaques.

中文翻译：

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用氟玉检测漂浮的脑部神经元变性和淀粉样β积累的替代方法。

Fluoro-Jade是一种荧光素衍生的荧光染料，可特异性结合受损的神经元。由于此特性，它通常用于组织化学检测和定量安装的脑部区域的神经变性。在这里，我们描述了一种基于自由浮动的脑部切片的替代方法和更简单的组织化学方案。为此，我们使用了野生型和5xFAD小鼠以及接受脑内注射寡聚淀粉样β肽的小鼠的脑切片。我们观察到，我们的组织化学染色程序允许对退化的神经元进行明确定义的标记，从而提供比常用的更好的信噪比染色。此外，我们修改后的方案证明了Fluoro-Jade C也可以荧光标记淀粉样蛋白β斑块的能力。