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Microwave diathermy induces mitogen-activated protein kinases and tumor necrosis factor-α in cultured human monocytes
Electromagnetic Biology and Medicine ( IF 1.7 ) Pub Date : 2019-05-11 , DOI: 10.1080/15368378.2019.1613426
George Lagoumintzis 1 , Andreas Andrikopoulos 1, 2 , Adam Adamopoulos 2 , Ioannis Seimenis 2 , Constantinos Koutsojannis 1
Affiliation  

ABSTRACT Although rehabilitation practice for most patients consists of a combined use of thermotherapy that is produced from diathermy devices resulting faster and deeper heating to the patient, major concerns about occupational exposure to electromagnetic radiation for the operators must be considered. In most occasions, physiotherapists have involved multi-hour treatment sessions to different patients, resulting overuse of the diathermy device. Recently, our team along with other groups have raised serious concerns about the occupational safety aspects related to microwave diathermy (MWD) use. Driven by these recent reports, in this work, we tried to investigate the in vitro effects of a physiotherapist routine MWD device regarding its potential inflammatory biological effects that could be evoked in human cultured monocytes. Our results show that MWD does not alter the integrity of the cell membrane and, consequently, the viability of monocytes as assessed by Trypan blue and MTT measurements. Then again, members of the MAPK family (p38 and ERK1/2) were activated upon MWD exposure at 5–30 min, eventually leading to a time-dependent considerable increase in TNF-α production, a key pro-inflammatory mediator. Our results are indicative of a stress-activated phenomenon of monocytes upon MWD radiation, which could trigger potential hazardous cellular outcomes due to thermal and/or non-thermal bystander effects. Our results deserve further investigation, planned by our team in due course, to delineate the clinical correlations of these findings.

中文翻译:

微波透热法在培养的人单核细胞中诱导丝裂原活化蛋白激酶和肿瘤坏死因子-α

摘要 虽然大多数患者的康复实践包括结合使用由透热设备产生的热疗,从而为患者提供更快和更深的加热,但必须考虑对操作者职业暴露于电磁辐射的主要担忧。在大多数情况下,物理治疗师对不同的患者进行了数小时的治疗,导致过度使用透热设备。最近,我们的团队与其他团体一起对与微波透热疗法 (MWD) 使用相关的职业安全问题提出了严重关切。在这些最近的报告的推动下,在这项工作中,我们试图研究物理治疗师常规 MWD 设备的体外效应,即可能在人类培养的单核细胞中引起的潜在炎症生物学效应。我们的结果表明 MWD 不会改变细胞膜的完整性,因此不会改变由台盼蓝和 MTT 测量评估的单核细胞的活力。然后,MAPK 家族的成员(p38 和 ERK1/2)在 MWD 暴露 5-30 分钟后被激活,最终导致 TNF-α 产生的时间依赖性显着增加,TNF-α 是一种关键的促炎介质。我们的结果表明单核细胞在 MWD 辐射时会出现应激激活现象,这可能会由于热和/或非热旁观者效应而引发潜在的危险细胞结果。我们的结果值得进一步调查,由我们的团队在适当的时候计划,以描述这些发现的临床相关性。通过台盼蓝和 MTT 测量评估单核细胞的活力。然后,MAPK 家族的成员(p38 和 ERK1/2)在 MWD 暴露 5-30 分钟后被激活,最终导致 TNF-α 产生的时间依赖性显着增加,TNF-α 是一种关键的促炎介质。我们的结果表明单核细胞在 MWD 辐射时会出现应激激活现象,这可能会由于热和/或非热旁观者效应而引发潜在的危险细胞结果。我们的结果值得进一步调查,由我们的团队在适当的时候计划,以描述这些发现的临床相关性。通过台盼蓝和 MTT 测量评估单核细胞的活力。然后,MAPK 家族的成员(p38 和 ERK1/2)在 MWD 暴露 5-30 分钟后被激活,最终导致 TNF-α 产生的时间依赖性显着增加,TNF-α 是一种关键的促炎介质。我们的结果表明单核细胞在 MWD 辐射时会出现应激激活现象,这可能会由于热和/或非热旁观者效应而引发潜在的危险细胞结果。我们的结果值得进一步调查,由我们的团队在适当的时候计划,以描述这些发现的临床相关性。一种关键的促炎介质。我们的结果表明单核细胞在 MWD 辐射时会出现应激激活现象,这可能会由于热和/或非热旁观者效应而引发潜在的危险细胞结果。我们的结果值得进一步调查,由我们的团队在适当的时候计划,以描述这些发现的临床相关性。一种关键的促炎介质。我们的结果表明单核细胞在 MWD 辐射时会出现应激激活现象,这可能会由于热和/或非热旁观者效应而引发潜在的危险细胞结果。我们的结果值得进一步调查,由我们的团队在适当的时候计划,以描述这些发现的临床相关性。
更新日期:2019-05-11
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