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Induced pluripotent stem cell-derived mesenchymal stem cell seeding on biofunctionalized calcium phosphate cements.
Bone Research ( IF 12.7 ) Pub Date : 2013-01-01 , DOI: 10.4248/br201304008
WahWah TheinHan 1 , Jun Liu 2 , Minghui Tang 1 , Wenchuan Chen 2 , Linzhao Cheng 3 , Hockin H K Xu 4
Affiliation  

Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferati on and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34+ cells which were reprogrammed by a single episomal vector. iPSCs were cultured to form embryoid bodies (EBs), and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC: RGD (Arg-Gly-Asp) peptides, fibronectin (Fn), fibronectin-like engineered polymer protein (FEPP), extracellular matrix Geltrex, and platelet concentrate. iPSC-MSCs were seeded on five biofunctionalized CPCs: CPC-RGD, CPC-Fn, CPC-FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and collagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering. iPSC-MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/orthopedic repairs.

中文翻译:

诱导多能干细胞衍生的间充质干细胞接种在生物功能化磷酸钙水泥上。

诱导多能干细胞 (iPSC) 由于其增殖和分化能力而具有巨大的潜力。本研究的目的是产生 iPSC 来源的间充质干细胞 (iPSC-MSCs),并首次研究 iPSC-MSC 在含有生物功能剂的磷酸钙骨水泥 (CPC) 上的增殖和成骨分化。人 iPSC 源自骨髓 CD34+ 细胞,这些细胞被单个游离型载体重编程。iPSCs 被培养形成胚状体 (EBs),MSCs 迁移出 EBs。CPC 中加入了五种生物功能剂:RGD (Arg-Gly-Asp) 肽、纤连蛋白 (Fn)、纤连蛋白样工程聚合物蛋白 (FEPP)、细胞外基质 Geltrex 和血小板浓缩物。iPSC-MSCs 接种在五种生物功能化的 CPCs 上:CPC-RGD、CPC-Fn、CPC-FEPP、CPC-Geltrex、和CPC-血小板。与对照相比,生物功能性 CPC 上的 iPSC-MSC 具有增强的增殖、肌动蛋白纤维表达、成骨分化和矿化。细胞增殖在生物功能 CPC 上大大增加。iPSC-MSC 经历了成骨分化,碱性磷酸酶、Runx2 和胶原蛋白-I 表达增加。iPSC-MSCs 在 CPC-Platelets 上的矿物质合成是 CPC 对照的 3 倍。总之,iPSCs 在骨工程方面显示出很高的潜力。生物功能化 CPC 上的 iPSC-MSC 具有比传统 CPC 更好的细胞增殖和骨矿化。iPSC-MSC-CPC 结构有望促进颅面/骨科修复中的骨再生。与对照相比,成骨分化和矿化。细胞增殖在生物功能 CPC 上大大增加。iPSC-MSC 经历了成骨分化,碱性磷酸酶、Runx2 和胶原蛋白-I 表达增加。iPSC-MSCs 在 CPC-Platelets 上的矿物质合成是 CPC 对照的 3 倍。总之,iPSCs 在骨工程方面显示出很高的潜力。生物功能化 CPC 上的 iPSC-MSC 具有比传统 CPC 更好的细胞增殖和骨矿化。iPSC-MSC-CPC 结构有望促进颅面/骨科修复中的骨再生。与对照相比,成骨分化和矿化。细胞增殖在生物功能 CPC 上大大增加。iPSC-MSC 经历了成骨分化,碱性磷酸酶、Runx2 和胶原蛋白-I 表达增加。iPSC-MSCs 在 CPC-Platelets 上的矿物质合成是 CPC 对照的 3 倍。总之,iPSCs 在骨工程方面显示出很高的潜力。生物功能化 CPC 上的 iPSC-MSC 具有比传统 CPC 更好的细胞增殖和骨矿化。iPSC-MSC-CPC 结构有望促进颅面/骨科修复中的骨再生。iPSC-MSCs 在 CPC-Platelets 上的矿物质合成是 CPC 对照的 3 倍。总之,iPSCs 在骨工程方面显示出很高的潜力。生物功能化 CPC 上的 iPSC-MSC 具有比传统 CPC 更好的细胞增殖和骨矿化。iPSC-MSC-CPC 结构有望促进颅面/骨科修复中的骨再生。iPSC-MSCs 在 CPC-Platelets 上的矿物质合成是 CPC 对照的 3 倍。总之,iPSCs 在骨工程方面显示出很高的潜力。生物功能化 CPC 上的 iPSC-MSC 具有比传统 CPC 更好的细胞增殖和骨矿化。iPSC-MSC-CPC 结构有望促进颅面/骨科修复中的骨再生。
更新日期:2019-11-01
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