During development, neuroepithelial progenitors acquire apico-basal polarity and adhere to one another via apically located tight and adherens junction complexes. This polarized neuroepithelium must continue to integrate cells arising through cell divisions and intercalation, and allow for cell movements, at the same time as undergoing morphogenesis. Cell proliferation, migration and intercalation all occur in the morphing embryonic eye. To understand how eye development might depend on dynamic epithelial adhesion, we investigated the function of a known regulator of junctional plasticity, Tumour necrosis factor receptor-associated factor 4 (Traf4). traf4a mRNA is expressed in the developing eye vesicle over the period of optic cup morphogenesis, and Traf4a loss leads to disrupted evagination and elongation of the eye vesicles, and aberrant organization and apico-basal polarity of the eye epithelium. We propose a model whereby Traf4a regulates apical junction plasticity in nascent eye epithelium, allowing for its polarization and morphogenesis. Symbols and Abbreviations: AB: apico-basal; aPKC: atypical protein kinase-C; CRISPR: clustered regularly-interspaced short palindromic repeats; GFP: green fluorescent protein; hpf: hours post-fertilization; MO: antisense morpholino oligonucleotide; pHH3: phospho histone H3; ss: somite stage; Traf4: Tumour necrosis factor receptor-associated factor 4; ZO-1: zona occludens-1.

中文翻译：

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眼上皮的极性和形态发生需要粘附连接相关的衔接蛋白Traf4。

在发育过程中，神经上皮祖细胞获得apico-基底极性，并通过顶端定位的紧密和粘附的连接复合物彼此粘附。这种极化的神经上皮细胞必须继续整合通过细胞分裂和嵌入产生的细胞，并允许细胞运动，同时进行形态发生。细胞增殖，迁移和嵌入都发生在变形的胚胎眼中。为了了解眼睛的发育可能取决于动态的上皮粘连，我们研究了已知的结缔合可调节因子，肿瘤坏死因子受体相关因子4（Traf4）的功能。traf4a mRNA在视杯形态发生期间在发育中的眼囊中表达，而Traf4a的丢失会导致眼囊的逃避和伸长受到破坏，以及眼上皮的异常组织和顶基极性。我们提出了一个模型，其中Traf4a调节新生眼上皮中的根尖可塑性，从而使其极化和形态发生。符号和缩写：AB：apico-basal；aPKC：非典型蛋白激酶C；CRISPR：成簇的规则间隔的短回文重复序列；GFP：绿色荧光蛋白；hpf：受精后数小时；MO：反义吗啉代寡核苷酸；pHH3：磷酸组蛋白H3；ss：somite阶段；Traf4：肿瘤坏死因子受体相关因子4；ZO-1：透明带遮盖物-1。非典型蛋白激酶C CRISPR：成簇的规则间隔的短回文重复序列；GFP：绿色荧光蛋白；hpf：受精后数小时；MO：反义吗啉代寡核苷酸；pHH3：磷酸组蛋白H3；ss：somite阶段；Traf4：肿瘤坏死因子受体相关因子4；ZO-1：透明带遮盖物-1。非典型蛋白激酶C CRISPR：成簇的规则间隔的短回文重复序列；GFP：绿色荧光蛋白；hpf：受精后数小时；MO：反义吗啉代寡核苷酸；pHH3：磷酸组蛋白H3；ss：somite阶段；Traf4：肿瘤坏死因子受体相关因子4；ZO-1：透明带遮盖物-1。