Abstract The application of quantum dots (QDs) as labels in immunoassay microarrays for the multiplex detection of 3‐phenoxybenzoic acid (PBA) and atrazine‐mercapturate (AM) has been demonstrated. PBA and AM are biomarkers of exposure to the pyrethroid insecticides and to the herbicide atrazine, respectively. Microarrays were fabricated by microcontact printing of the coating antigens in line patterns onto glass substrates. Competitive immunoassays were successfully performed using QDs (QD560 and QD620) as reporters. The multiplexed immunoassays were characterized by fluorescence microscopy and SEM. The application of QD fluorophores facilitates multiplex assays and therefore can contribute to enhanced throughput in biomonitoring.

中文翻译：

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量子点在多重免疫测定中作为暴露于农用化学品的生物标志物的报告基因

摘要 量子点 (QD) 作为标记物在免疫分析微阵列中的应用已被证明，用于多重检测 3-苯氧基苯甲酸 (PBA) 和莠去津-巯基 (AM)。PBA 和 AM 分别是暴露于拟除虫菊酯杀虫剂和除草剂阿特拉津的生物标志物。微阵列是通过将涂层抗原以线条图案微接触印刷到玻璃基板上来制造的。使用 QD（QD560 和 QD620）作为报告基因成功地进行了竞争性免疫测定。多重免疫测定的特征在于荧光显微镜和扫描电镜。QD 荧光团的应用促进了多重检测，因此有助于提高生物监测的通量。