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Evaluation of methanotrophic bacterial communities capable of biodegrading trichloroethene (TCE) in acidic aquifers.
Biodegradation ( IF 3.6 ) Pub Date : 2019-04-11 , DOI: 10.1007/s10532-019-09875-w
Yiru Shao 1 , Paul B Hatzinger 2 , Sheryl H Streger 2 , Rachael T Rezes 2 , Kung-Hui Chu 1
Affiliation  

Abstract

While bioremediation technologies for trichloroethene (TCE), a suspected carcinogen, have been successfully demonstrated in neutral pH aquifers, these technologies are often ineffective for remediating TCE contamination in acidic aquifers (i.e., pH < 5.5). Acidophilic methanotrophs have been detected in several low pH environments, but their presence and potential role in TCE degradation in acidic aquifers is unknown. This study applied a stable isotope probing-based technique to identify active methanotrophs that are capable of degrading TCE in microcosms prepared from two low pH aquifers. A total of thirty-five clones of methanotrophs were derived from low pH microcosms in which methane and TCE degradation had been observed, with 29 clustered in γ-Proteobacteria and 6 clustered in α-Proteobacteria. None of the clones has a high similarity to known acidophilic methanotrophs from other environments. The presence and diversity of particulate MMO and soluble MMO were also investigated. The pmoA gene was detected predominantly at one site, and the presence of a specific form of mmoX in numerous samples suggested that Methylocella spp. may be common in acidic aquifers. Finally, a methane-grown culture at pH 4 was enriched from an acidic aquifer and its ability to biodegrade various chlorinated ethenes was tested. Interestingly, the mixed culture rapidly degraded TCE and vinyl chloride, but not cis-dichloroethene after growth on methane. The data suggest that aerobic biodegradation of TCE and other chlorinated solvents in low pH groundwater may be facilitated by methanotrophic bacteria, and that there are potentially a wide variety of different strains that inhabit acidic aquifers.

Graphical abstract



中文翻译:

能够在酸性含水层中生物降解三氯乙烯(TCE)的甲烷营养细菌群落的评估。

摘要

尽管已在中性pH含水层中成功证明了可疑致癌物三氯乙烯(TCE)的生物修复技术,但这些技术通常无法有效地补救酸性含水层中的TCE污染(即pH <5.5)。在几种低pH环境中已检测到嗜酸性甲烷氧化菌,但在酸性含水层中TCE降解的存在及其潜在作用尚不清楚。这项研究应用了基于稳定同位素探测的技术,以鉴定能够降解由两个低pH含水层制备的微观世界中TCE的活性甲烷营养生物。共有35个甲烷营养菌的克隆来自低pH的微观世界,其中观察到甲烷和TCE降解,其中29个聚集在γ-变形杆菌中,6个聚集在α-细菌中。变形杆菌。没有一个克隆与其他环境中已知的嗜酸性甲烷营养生物具有高度相似性。还研究了颗粒MMO和可溶性MMO的存在和多样性。所述pmoA在一个部位检测到的主要的基因,和一种特定形式的存在mmoX众多样品中建议Methylocella属。在酸性含水层中可能很常见。最后,从酸性含水层中富集了甲烷生长的pH为4的培养物,并测试了其生物降解各种氯化乙烯的能力。有趣的是,混合培养物迅速降解了TCE和氯乙烯,但没有降解出顺式在甲烷上生长后的-二氯乙烯。数据表明,甲烷营养细菌可促进低pH值地下水中TCE和其他氯化溶剂的好氧生物降解,并且可能有各种各样的菌株生活在酸性含水层中。

图形概要

更新日期:2019-04-11
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