Biodegradation of 3,5,6-trichloro-2-pyridinol (TCP) in drylands is an important biological process of detoxification. Flooding in drylands can result in the formation of anaerobic habitats. However, little is known about the microbial metabolism of TCP in dryland soil under anaerobic conditions. Here, chlorpyrifos-contaminated dryland soil was incubated to enrich the TCP-degrading microbial consortium under anaerobic conditions. Chloridion and CO₂ were released with TCP degradation, and the enrichment cultures of dryland soil could metabolize 97% of TCP (100 mg/L) within 20 h. Both reductive and hydrolysis dechlorination mechanisms were involved in TCP biodegradation under anaerobic conditions. Bacterial taxonomic analysis revealed that the aerobic TCP-degrading bacteria Ochrobactrum and dechlorination bacteria Delftia were the dominant genera. Anaerobic and facultative bacteria; i.e., Bacteroides, Bacillus, and Cupriavidus had lower relative abundances, but they were significantly enriched following treatment with TCP. These results indicate that the enrichment cultures of dryland soil dominated by aerobic bacteria could dechlorinate and degrade TCP under anaerobic conditions.

中文翻译：

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厌氧培养条件下微生物群落对旱地土壤中3,5,6-三氯-2-吡啶醇的降解。

旱地3,5,6-三氯-2-吡啶醇（TCP）的生物降解是重要的解毒生物学过程。旱地的洪水会导致厌氧生境的形成。但是，关于厌氧条件下旱地土壤中TCP的微生物代谢知之甚少。在这里，受毒死rif污染的旱地土壤在厌氧条件下进行培养，以富集TCP降解微生物群落。随着TCP的降解释放出氯和CO ₂，旱地土壤的富集培养在20h内可以代谢97％的TCP（100 mg / L）。在厌氧条件下，TCP的生物降解涉及还原和水解脱氯机理。细菌分类学分析表明，需氧降解TCP的细菌是ch和脱氯细菌德尔夫菌是主要的属。厌氧和兼性细菌；即，拟杆菌，芽孢杆菌和铜杯虫的相对丰度较低，但在用TCP处理后它们明显富集。这些结果表明，在好氧条件下，以好氧细菌为主的旱地土壤的富集培养可以使TCP脱氯并降解。