Versican is a large chondroitin sulfate proteoglycan enriched in the extracellular matrix, and it has at least four different isoforms, termed V0, V1, V2, and V3. Although several studies have demonstrated that versican is stably expressed in various developing organs, the expression of versican isoforms during tooth development has not been elucidated yet. Therefore, the present study was to investigate the expression of versican isoforms in the developing mouse molars. The mandibular first molars from embryonic day (E) 11.5 to postnatal day (PN) 21 were used to investigate the expression of versican isoforms by immunohistochemistry, and the gene expressions of versican (Vcan) isoforms from E13.5 to PN7 were analyzed by quantitative real-time PCR. The results exhibited different expressing patterns of versican isoforms-the stellate reticulum (SR) and the dental mesenchymal cells adjacent to Hertwig's Epithelial Root Sheath (HERS) only expressed V1 and the mature odontoblasts mainly expressed V2, while the dental papilla and the ameloblasts might both express V0/V1/V2. These results suggested that different versican isoforms may act different roles in the tooth development, and we speculated that V0/V1 might be intimately involved in the cell proliferation while V2 was associated in the cytodifferentiation.

中文翻译：

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小鼠下颌第一磨牙的发育过程中，Versican的不同亚型的表达。

Versican是富含细胞外基质的大型硫酸软骨素蛋白聚糖，它具有至少四个不同的亚型，称为V0，V1，V2和V3。尽管几项研究表明，凡是在各种发育器官中稳定表达的，但尚未阐明在牙齿发育过程中其同种型的表达。因此，本研究旨在研究versican同工型在发育中的小鼠磨牙中的表达。应用免疫组织化学方法研究从胚胎第（E）11.5天至出生后第（PN）21天的下颌第一磨牙的Verscan亚型表达，并定量分析E13.5至PN7的Verscanan（Vcan）亚型的基因表达。实时PCR。结果显示出不同的Verscan亚型表达模式-星状网（SR）和与Hertwig上皮根鞘（HERS）相邻的牙齿间充质细胞仅表达V1，成熟的成牙本质细胞主要表达V2，而牙乳头和成釉细胞可能都表达表示V0 / V1 / V2。这些结果表明，不同的versican同工型可能在牙齿发育中发挥不同的作用，我们推测V0 / V1可能与细胞增殖密切相关，而V2与细胞分化有关。