The extracellular matrix proteoglycan decorin is well-known for its oncosuppressive activity. Here, decorin expression was examined in human vulva carcinoma tissue samples and in primary and commercial cell lines representing this malignant disease. Furthermore, the effect of adenovirus-mediated decorin cDNA (Ad-DCN) transduction on the viability, proliferation, and the expression and activity of the epidermal growth factor receptor (ErbB/HER) family members of the cell lines were investigated. Using in situ hybridization and immunohistochemistry for decorin, it was demonstrated that malignant cells in human vulva carcinoma tissues lack decorin expression. This result was true independently on tumor stage, grade or human papillomavirus status. RT-qPCR analyses showed that the human vulva carcinoma cell lines used in this study were also negative for decorin expression. Transduction of the cell lines with Ad-DCN caused a marked reduction in cell viability, while the proliferation of the cells was not affected. Experiments examining potential mechanisms behind the oncosuppressive effect of Ad-DCN transduction revealed that ErbB2/HER2 expression and activity in carcinoma cells were markedly downregulated. In conclusion, the results of this study showed that human vulva carcinoma cells lack decorin expression, and that Ad-DCN transduction of these cells induces oncosuppressive activity in part via downregulation of ErbB2/HER2.

中文翻译：

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Decorin在外阴癌中的表达：Decorin cDNA转导对癌细胞的抑癌作用。

细胞外基质蛋白聚糖decorin以其抑癌活性而闻名。在这里，在人的外阴癌组织样品以及代表这种恶性疾病的原代和商业细胞系中检查了除芯蛋白的表达。此外，研究了腺病毒介导的decorin cDNA（Ad-DCN）转导对细胞系的表皮生长因子受体（ErbB / HER）家族的活力，增殖以及表达和活性的影响。使用原位杂交和免疫组化的decorin，已证明人类外阴癌组织中的恶性细胞缺乏decorin表达。该结果独立于肿瘤的阶段，等级或人乳头瘤病毒状态是正确的。RT-qPCR分析表明，本研究中使用的人外阴癌细胞株对decorin表达也呈阴性。用Ad-DCN转导细胞系引起细胞活力的显着降低，而细胞的增殖不受影响。实验检查了Ad-DCN转导的抑癌作用背后的潜在机制，发现癌细胞中ErbB2 / HER2的表达和活性显着下调。总之，这项研究的结果表明，人类外阴癌细胞缺乏核心蛋白表达，Ad-DCN转导这些细胞部分通过下调ErbB2 / HER2诱导癌抑制活性。实验检查了Ad-DCN转导的抑癌作用背后的潜在机制，发现癌细胞中ErbB2 / HER2的表达和活性显着下调。总之，这项研究的结果表明，人类外阴癌细胞缺乏核心蛋白表达，Ad-DCN转导这些细胞部分通过下调ErbB2 / HER2诱导癌抑制活性。实验检查了Ad-DCN转导的抑癌作用背后的潜在机制，发现癌细胞中ErbB2 / HER2的表达和活性显着下调。总之，这项研究的结果表明，人类外阴癌细胞缺乏核心蛋白表达，Ad-DCN转导这些细胞部分通过下调ErbB2 / HER2诱导癌抑制活性。