Metabolomics is a rapidly developing branch of science that concentrates on identifying biologically active molecules with potential biomarker properties. To define the best biomarkers for diseases, metabolomics uses both models (in vitro, animals) and human, as well as, various techniques such as mass spectroscopy, gas chromatography, liquid chromatography, infrared and UV–VIS spectroscopy and nuclear magnetic resonance. The last one takes advantage of the magnetic properties of certain nuclei, such as 1H, 13C, 31P, 19F, especially their ability to absorb and emit energy, what is crucial for analyzing samples. Among many spectroscopic NMR techniques not only one-dimensional (1D) techniques are known, but for many years two-dimensional (2D, for example, COSY, DOSY, JRES, HETCORE, HMQS), three-dimensional (3D, DART-MS, HRMAS, HSQC, HMBC) and solid-state NMR have been used. In this paper, authors taking apart fundamental division of nuclear magnetic resonance techniques intend to shown their wide application in metabolomic studies, especially in identifying biomarkers.

中文翻译：

---

代谢组学研究中的核磁共振技术：利用实例的快速概述

代谢组学是一个快速发展的科学分支，专注于识别具有潜在生物标志物特性的生物活性分子。为了定义疾病的最佳生物标志物，代谢组学使用模型（体外、动物）和人类模型，以及各种技术，如质谱、气相色谱、液相色谱、红外和紫外-可见光谱以及核磁共振。最后一种利用了某些原子核的磁性，例如 1H、13C、31P、19F，特别是它们吸收和发射能量的能力，这对于分析样品至关重要。在许多光谱 NMR 技术中，不仅已知一维 (1D) 技术，而且多年来已知二维（2D，例如 COSY、DOSY、JRES、HETCORE、HMQS）、三维（3D、DART-MS , 人力资源管理系统, HSQC, HMBC）和固态核磁共振已被使用。在本文中，作者将核磁共振技术的基本划分分开，旨在展示它们在代谢组学研究中的广泛应用，特别是在识别生物标志物方面。