The Streptomyces sp. strain AV05 isolated from an organic amendment was found to impact both growth and fumonisin production of Fusarium verticillioides during in vitro direct confrontation. In order to investigate the interactions between the Streptomyces sp. strain AV05 and F. verticillioides, a metabolomic approach was used. The study of the endometabolomes of the microorganisms was carried out in two different conditions: the microorganisms were cultivated alone or in confrontation. The aim of this study was to examine the modifications of the endometabolome of F. verticillioides in confrontation with the Streptomyces strain. The metabolites involved in these modifications were identified using 2D NMR. Many metabolites were found to be overproduced in confrontation assays with the Streptomyces strain, notably 16 proteinogenic amino acids, inosine, and uridine. This suggested that fungal metabolic pathways such as protein synthesis have been affected due to interaction. Thus, metabolomic studies, as well as proteomics or transcriptomics, are useful for deciphering the mechanisms of interactions between biological control agents and mycotoxigenic fungi. This comprehension is one of the key elements of the improvement of the selection and use of antagonistic agents.

中文翻译：

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枯萎镰刀菌与链霉菌种体外相互作用的研究。使用代谢组学。

链霉菌 发现从有机修饰物中分离出的AV05菌株在体外直接对抗过程中会影响褐斑镰刀菌的生长和伏马菌素的生产。为了调查链霉菌属之间的相互作用。菌株AV05和F. verticillioides，使用代谢组学方法。在两种不同条件下进行了微生物内生菌的研究：单独培养或对抗培养微生物。这项研究的目的是检查与链霉菌菌株对立的拟南芥内膜代谢组的修饰。使用2D NMR鉴定了参与这些修饰的代谢物。在链霉菌菌株的对抗试验中发现许多代谢产物都过量产生，特别是16种蛋白原氨基酸，肌苷和尿苷。这表明诸如蛋白质合成的真菌代谢途径由于相互作用而受到影响。因此，代谢组学研究以及蛋白质组学或转录组学，对于破译生物控制剂和产毒真菌之间的相互作用机制非常有用。这种理解是改善拮抗药选择和使用的关键要素之一。