This report summarises the presentations and activities of the ISEV Workshop on extracellular vesicle biomarkers held in Birmingham, UK during December 2017. Among the key messages was broad agreement about the importance of biospecimen science. Much greater attention needs to be paid towards the provenance of collected samples. The workshop also highlighted clear gaps in our knowledge about pre-analytical factors that alter extracellular vesicles (EVs). The future utility of certified standards for credentialing of instruments and software, to analyse EV and for tracking the influence of isolation steps on the structure and content of EVs were also discussed. Several example studies were presented, demonstrating the potential utility for EVs in disease diagnosis, prognosis, longitudinal serial testing and stratification of patients. The conclusion of the workshop was that more effort focused on pre-analytical issues and benchmarking of isolation methods is needed to strengthen collaborations and advance more effective biomarkers.

本报告总结了 2017 年 12 月在英国伯明翰举行的 ISEV 细胞外囊泡生物标志物研讨会的报告和活动。关键信息包括对生物样本科学重要性的广泛共识。需要更加关注采集样本的来源。研讨会还强调了我们对改变细胞外囊泡 (EV) 的分析前因素的认识存在明显差距。还讨论了认证标准在仪器和软件认证、分析 EV 以及跟踪隔离步骤对 EV 结构和内容的影响方面的未来效用。介绍了几个示例研究，证明了 EV 在疾病诊断、预后、纵向系列测试和患者分层中的潜在效用。