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Recent advances in spontaneous Raman spectroscopic imaging: instrumentation and applications.
Current Medicinal Chemistry ( IF 4.1 ) Pub Date : 2020-10-31 , DOI: 10.2174/0929867326666190619114431
Nan Wang 1, 2 , Honghao Cao 1, 2 , Lin Wang 3 , Feng Ren 1, 2 , Qi Zeng 1, 2 , Xinyi Xu 1, 2 , Jimin Liang 1, 2 , Yonghua Zhan 1, 2 , Xueli Chen 1, 2
Affiliation  

Background: Spectroscopic imaging based on the spontaneous Raman scattering effects can provide unique fingerprint information in relation to the vibration bands of molecules. Due to its advantages of high chemical specificity, non-invasive detection capability, low sensitivity to water, and no special sample pretreatment, Raman Spectroscopic Imaging (RSI) has become an invaluable tool in the field of biomedicine and medicinal chemistry.

Methods: There are three methods to implement RSI, including point scanning, line scanning and wide-field RSI. Point-scanning can achieve two-and three-dimensional imaging of target samples. High spectral resolution, full spectral range and confocal features render this technique highly attractive. However, point scanning based RSI is a time-consuming process that can take several hours to map a small area. Line scanning RSI is an extension of point scanning method, with an imaging speed being 300-600 times faster. In the wide-field RSI, the laser illuminates the entire region of interest directly and all the images then collected for analysis. In general, it enables more accurate chemical imaging at faster speeds.

Results: This review focuses on the recent advances in RSI, with particular emphasis on the latest developments on instrumentation and the related applications in biomedicine and medicinal chemistry. Finally, we prospect the development trend of RSI as well as its potential to translation from bench to bedside.

Conclusion: RSI is a powerful technique that provides unique chemical information, with a great potential in the fields of biomedicine and medicinal chemistry.



中文翻译:

自发拉曼光谱成像的最新进展:仪器和应用。

背景:基于自发拉曼散射效应的光谱成像可以提供有关分子振动带的独特指纹信息。由于拉曼光谱成像(RSI)具有高化学特异性,无创检测能力,对水的敏感性低以及无需特殊样品预处理的优点,因此已成为生物医学和药物化学领域的宝贵工具。

方法:有三种实现RSI的方法,包括点扫描,线扫描和广域RSI。点扫描可以实现目标样本的二维和三维成像。高光谱分辨率,全光谱范围和共焦特性使该技术极具吸引力。但是,基于点扫描的RSI是一个耗时的过程,可能需要几个小时才能绘制出一个小区域。线扫描RSI是点扫描方法的扩展,其成像速度提高了300-600倍。在宽视场RSI中,激光直接照射整个感兴趣区域,然后收集所有图像进行分析。通常,它可以更快的速度实现更精确的化学成像。

结果:这篇综述着重于RSI的最新进展,特别着重于仪器的最新发展以及在生物医学和药物化学中的相关应用。最后,我们展望了RSI的发展趋势及其从工作台到床边翻译的潜力。

结论:RSI是一项功能强大的技术,可提供独特的化学信息,在生物医学和药物化学领域具有巨大的潜力。

更新日期:2020-11-05
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