Oncology research projects are highly dependent on the quality of tumor samples stored in the biobank. Microscopic control is important to ensure the quality of the frozen sample (Does the sample correspond to tumor tissue? Does the sample contain a sufficient number of tumor cells for molecular analysis?). The aim of this study was to evaluate the value of the mirror image method in quality control of colonic adenocarcinoma samples stored in a tumor bank. Microscopic concordance for the differentiation grade, malignant and normal cell percentages, necrosis, mucinous component, and ulceration was assessed on 82 colon adenocarcinoma banked samples and their paired, formalin-fixed, paraffin-embedded mirror controls. Molecular concordance for KRAS status was evaluated in 76 of these 82 cases. Morphological correspondence between frozen and mirror samples was good for the mucinous component (intraclass correlation coefficient [ICC] = 0.81), moderate for differentiation (Cohen's kappa coefficient [k] = 0.67), fair for malignant cells (ICC = 0.44), and poor for ulceration (k = 0.08), normal tissue (ICC = 0.36), and necrosis (ICC = 0.13) percentages. Molecular correspondence for KRAS status was almost perfect (95% correspondence, k = 0.88) between frozen and mirror samples. In conclusion, the mirror sample method is not a good alternative for microscopic and molecular control of frozen colonic adenocarcinoma samples.

中文翻译：

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镜像法在肿瘤组织库质量控制中真的有用吗？

肿瘤学研究项目高度依赖于生物库中存储的肿瘤样品的质量。显微镜控制对于确保冷冻样品的质量很重要（样品是否对应于肿瘤组织？样品是否包含足够数量的肿瘤细胞用于分子分析？）。这项研究的目的是评估镜像方法在肿瘤库中存储的结肠腺癌样品质量控制中的价值，并评估了分化程度，恶性和正常细胞百分比，坏死，粘液成分和溃疡的微观一致性。对82例结肠腺癌库存样品及其配对，福尔马林固定，石蜡包埋的镜对照进行了研究。在这82例病例中，有76例评估了KRAS状态的分子一致性。冷冻样品和镜检样品之间的形态学对应性对粘液成分良好（类内相关系数[ICC] = 0.81），对分化适度（Cohen卡伯系数[k] = 0.67），对恶性细胞尚可（ICC = 0.44），差对于溃疡（k = 0.08），正常组织（ICC = 0.36）和坏死（ICC = 0.13）百分比。冷冻样品和镜像样品之间的KRAS状态分子对应关系几乎完美（95％对应关系，k = 0.88）。总之，对于冷冻结肠腺癌样品的显微镜和分子控制，镜面样品方法不是一个很好的选择。且溃疡率（k = 0.08），正常组织（ICC = 0.36）和坏死（ICC = 0.13）百分比差。冷冻样品和镜像样品之间的KRAS状态分子对应关系几乎完美（95％对应关系，k = 0.88）。总之，对于冷冻结肠腺癌样品的显微镜和分子控制，镜面样品方法不是一个很好的选择。且溃疡率（k = 0.08），正常组织（ICC = 0.36）和坏死（ICC = 0.13）百分比差。冷冻样品和镜像样品之间的KRAS状态分子对应关系几乎完美（95％对应关系，k = 0.88）。总之，对于冷冻结肠腺癌样品的显微镜和分子控制，镜面样品方法不是一个很好的选择。