BACKGROUND The determination of microbial communities using the mothur tool suite (https://www.mothur.org) is well established. However, mothur requires bioinformatics-based proficiency in order to perform calculations via the command-line. Galaxy is a project dedicated to providing a user-friendly web interface for such command-line tools (https://galaxyproject.org/). RESULTS We have integrated the full set of 125+ mothur tools into Galaxy as the Galaxy mothur Toolset (GmT) and provided a set of workflows to perform end-to-end 16S rRNA gene analyses and integrate with third-party visualization and reporting tools. We demonstrate the utility of GmT by analyzing the mothur MiSeq standard operating procedure (SOP) dataset (https://www.mothur.org/wiki/MiSeq_SOP). CONCLUSIONS GmT is available from the Galaxy Tool Shed, and a workflow definition file and full Galaxy training manual for the mothur SOP have been created. A Docker image with a fully configured GmT Galaxy is also available.

中文翻译：

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Galaxy mothur Toolset（GmT）：使用mothur进行16S rRNA基因测序分析的用户友好应用程序。

背景技术使用mothur工具套件（https://www.mothur.org）对微生物群落的确定是公认的。但是，mothur需要基于生物信息学的能力，才能通过命令行执行计算。Galaxy是一个致力于为此类命令行工具（https://galaxyproject.org/）提供用户友好的Web界面的项目。结果我们将全套125多种mothur工具集成为Galaxy mothur工具集（GmT），并提供了一组工作流来执行端到端16S rRNA基因分析，并与第三方可视化和报告工具集成。我们通过分析mothur MiSeq标准操作程序（SOP）数据集（https://www.mothur.org/wiki/MiSeq_SOP）证明了GmT的实用性。结论GmT可从Galaxy Tool Shed获得，并已创建用于mothur SOP的工作流程定义文件和完整的Galaxy培训手册。还提供带有完全配置的GmT Galaxy的Docker映像。