Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identification. Here, we use a modified version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (∼15 min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This field-ready SHERLOCK platform with color-based lateral flow readout can be applied for detection and quantitation of genes in a range of agricultural applications.

中文翻译：

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使用CRISPR-Cas13对植物基因进行核酸检测。

核酸检测对于农业应用至关重要，包括育种过程中的性状检测，害虫监视和病原体鉴定。在这里，我们使用基于CRISPR的核酸检测平台SHERLOCK的改良版来量化大豆混合物中草甘膦抗性基因的水平，并在单个反应中检测多个植物基因。SHERLOCK快速（〜15分钟），定量且便于携带，可以加工大豆粗提物作为输入材料，以最少的核酸样品制备。这种具有基于颜色的侧向流读数的现成的SHERLOCK平台可用于多种农业应用中的基因检测和定量。