BACKGROUND Blastomere movement (BMov) occurs after the first cell division in human embryos. This movement has been suggested as a prognostic parameter for pregnancy outcome prediction following cleavage-stage embryo transfer. However, the effect of BMov on preimplantation development and pregnancy outcome after blastocyst transfer remains unclear. Therefore, this study aimed to evaluate whether BMov after the first cell division is correlated with blastocyst formation rate and live birth rate after single vitrified-warmed blastocyst transfer (SVBT). METHODS Nine hundred and sixty-six embryos cultured in the EmbryoScope+® time-lapse system were retrospectively analyzed. The BMov type was categorized into three groups; namely, bouncing, wobbling, and twist-and-crumble. The BMov duration (dBMov) between the first (t2) and second cell division (t3) was monitored, and the ratio of dBMov to the duration of the 2-cell stage was calculated [dBMov/(t3-t2)]. Developmental rates to the 4-cell, 8-cell, morula, blastocyst, and expanded blastocyst stages were assessed, as well as blastocyst morphological grade. The correlations between dBMov and clinical pregnancy, ongoing pregnancy, and live birth rates were evaluated. RESULTS Increased dBMov/(t3-t2) was significantly correlated with decreased developmental rates to the 8-cell, morula, blastocyst, and expanded blastocyst stages, especially from the 4-cell stage to the morula stage. Analysis of different types of BMov revealed that embryos with bouncing movement exhibited significantly higher developmental rates to the 8-cell, morula, blastocyst, and expanded blastocyst stages compared with embryos with twist-and-crumble movement. The morphological quality of blastocyst-stage embryos with twist-and-crumble movement was significantly lower than that of embryos with bouncing and wobbling movements. The rates of clinical pregnancy, ongoing pregnancy, and live birth after SVBT were not correlated with BMov type or duration. CONCLUSIONS Embryonic compaction and subsequent blastocyst formation are adversely affected by twist-and-crumble movement and prolonged movement after the first cell division. Our results indicate that the preimplantation developmental competence of human embryos could be predicted by assessing BMov after the first cell division on day 1.

中文翻译：

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第一次细胞分裂后卵裂球的运动与胚胎紧实和随后的胚泡形成有关。

背景技术卵裂球运动（BMov）发生在人类胚胎中的第一次细胞分裂之后。该运动已被建议作为卵裂期胚胎移植后预测妊娠结局的预后参数。然而，BMov对胚泡转移后植入前发育和妊娠结局的影响尚不清楚。因此，本研究旨在评估第一个细胞分裂后的BMov是否与单玻璃化温热囊胚转移（SVBT）后的囊胚形成率和活产率相关。方法回顾性分析在EmbryoScope +®延时系统中培养的966个胚胎。BMov类型分为三类：即弹跳，摆动和扭曲崩溃。监测第一细胞分裂（t2）和第二细胞分裂（t3）之间的BMov持续时间（dBMov），并计算dBMov与2细胞阶段持续时间的比率[dBMov /（t3-t2）]。评估了4细胞，8细胞，桑ula，囊胚和扩大的囊胚阶段的发育率，以及囊胚的形态学等级。评估了dBMov与临床妊娠，正在进行的妊娠和活产率之间的相关性。结果增加的dBMov /（t3-t2）与8细胞，桑ula，囊胚和扩大的囊胚阶段，特别是从4细胞到桑ula胚阶段的发育率降低显着相关。对不同类型BMov的分析表明，具有弹跳运动的胚胎对8细胞，桑ula，胚泡，与具有扭曲和破碎运动的胚胎相比，胚泡期扩大了。具有扭转和破碎运动的胚泡期胚胎的形态学质量明显低于具有弹跳和摇摆运动的胚的形态质量。SVBT后的临床妊娠率，持续妊娠率和活产率与BMov类型或持续时间无关。结论第一次细胞分裂后，扭转和碎裂运动和长时间运动不利地影响了胚胎的紧实和随后的胚泡形成。我们的结果表明，可以通过评估第1天第一个细胞分裂后的BMov来预测人类胚胎的植入前发育能力。具有扭转和破碎运动的胚泡期胚胎的形态学质量明显低于具有弹跳和摇摆运动的胚的形态质量。SVBT后的临床妊娠率，持续妊娠率和活产率与BMov类型或持续时间无关。结论第一次细胞分裂后，扭转和碎裂运动和长时间运动不利地影响了胚胎的紧实和随后的胚泡形成。我们的结果表明，可以通过评估第1天第一个细胞分裂后的BMov来预测人类胚胎的植入前发育能力。具有扭转和破碎运动的胚泡期胚胎的形态学质量明显低于具有弹跳和摇摆运动的胚的形态质量。SVBT后的临床妊娠率，持续妊娠率和活产率与BMov类型或持续时间无关。结论第一次细胞分裂后，扭转和碎裂运动和长时间运动不利地影响了胚胎的紧实和随后的胚泡形成。我们的结果表明，可以通过评估第1天第一个细胞分裂后的BMov来预测人类胚胎的植入前发育能力。结论第一次细胞分裂后，扭转和碎裂运动和长时间运动不利地影响了胚胎的紧实和随后的胚泡形成。我们的结果表明，可以通过评估第1天第一个细胞分裂后的BMov来预测人类胚胎的植入前发育能力。结论第一次细胞分裂后，扭转和碎裂运动和长时间运动不利地影响了胚胎的紧实和随后的胚泡形成。我们的结果表明，可以通过评估第1天第一个细胞分裂后的BMov来预测人类胚胎的植入前发育能力。